A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins

C. E. Anderson; J. R. Buser; E. M. Strauch; R. Marzan; D. Baker; P. Yager

A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins

C. E. Anderson
, J. R. Buser
, E. M. Strauch
, R. Marzan
, D. Baker
, P. Yager

Division of Infectious Diseases

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Despite the ubiquitous nature of influenza, existing rapid diagnostics exhibit poor sensitivity and lack the ability to subtype [1]. We have developed a paper-based assay to target the viral surface protein hemagglutinin (HA) using computationally designed affinity proteins. This assay has been validated for the detection of two strains of the influenza virus spiked into influenza-negative nasal swab samples. Here, we present a device for the automation of this assay for specific detection of intact influenza virus. Using recent advances in paper microfluidics, we demonstrate that the integration of an enzymatic amplification technique using separate fluid paths can be used to enable high sensitivity on device.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society
Pages	968-969
Number of pages	2
ISBN (Electronic)	9780979806490
State	Published - 2016
Event	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: Oct 9 2016 → Oct 13 2016

Publication series

Name	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Conference

Conference	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	10/9/16 → 10/13/16

Keywords

Hemagglutinin
Influenza virus
Paper microfluidics
Protein binders

Cite this

Anderson, C. E., Buser, J. R., Strauch, E. M., Marzan, R., Baker, D., & Yager, P. (2016). A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 968-969). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.

Anderson, C. E. ; Buser, J. R. ; Strauch, E. M. et al. / A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 968-969 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

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title = "A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins",

abstract = "Despite the ubiquitous nature of influenza, existing rapid diagnostics exhibit poor sensitivity and lack the ability to subtype [1]. We have developed a paper-based assay to target the viral surface protein hemagglutinin (HA) using computationally designed affinity proteins. This assay has been validated for the detection of two strains of the influenza virus spiked into influenza-negative nasal swab samples. Here, we present a device for the automation of this assay for specific detection of intact influenza virus. Using recent advances in paper microfluidics, we demonstrate that the integration of an enzymatic amplification technique using separate fluid paths can be used to enable high sensitivity on device.",

keywords = "Hemagglutinin, Influenza virus, Paper microfluidics, Protein binders",

author = "Anderson, \{C. E.\} and Buser, \{J. R.\} and Strauch, \{E. M.\} and R. Marzan and D. Baker and P. Yager",

note = "Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01AI096184 and by the UW Department of Bioengineering.; 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

year = "2016",

language = "English",

series = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society",

pages = "968--969",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

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Anderson, CE, Buser, JR, Strauch, EM, Marzan, R, Baker, D & Yager, P 2016, A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 968-969, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 10/9/16.

A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins. / Anderson, C. E.; Buser, J. R.; Strauch, E. M. et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 968-969 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins

AU - Anderson, C. E.

AU - Buser, J. R.

AU - Strauch, E. M.

AU - Marzan, R.

AU - Baker, D.

AU - Yager, P.

N1 - Funding Information: This work was supported by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health under award number R01AI096184 and by the UW Department of Bioengineering.

PY - 2016

Y1 - 2016

N2 - Despite the ubiquitous nature of influenza, existing rapid diagnostics exhibit poor sensitivity and lack the ability to subtype [1]. We have developed a paper-based assay to target the viral surface protein hemagglutinin (HA) using computationally designed affinity proteins. This assay has been validated for the detection of two strains of the influenza virus spiked into influenza-negative nasal swab samples. Here, we present a device for the automation of this assay for specific detection of intact influenza virus. Using recent advances in paper microfluidics, we demonstrate that the integration of an enzymatic amplification technique using separate fluid paths can be used to enable high sensitivity on device.

AB - Despite the ubiquitous nature of influenza, existing rapid diagnostics exhibit poor sensitivity and lack the ability to subtype [1]. We have developed a paper-based assay to target the viral surface protein hemagglutinin (HA) using computationally designed affinity proteins. This assay has been validated for the detection of two strains of the influenza virus spiked into influenza-negative nasal swab samples. Here, we present a device for the automation of this assay for specific detection of intact influenza virus. Using recent advances in paper microfluidics, we demonstrate that the integration of an enzymatic amplification technique using separate fluid paths can be used to enable high sensitivity on device.

KW - Hemagglutinin

KW - Influenza virus

KW - Paper microfluidics

KW - Protein binders

UR - https://www.scopus.com/pages/publications/85014239869

M3 - Conference contribution

AN - SCOPUS:85014239869

T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

SP - 968

EP - 969

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society

T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Y2 - 9 October 2016 through 13 October 2016

ER -

Anderson CE, Buser JR, Strauch EM, Marzan R, Baker D, Yager P. A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 968-969. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

A two dimensional paper network for automated detection of the influenza virus employing computationally-designed affinity proteins

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