A trimming-and-retrieving alignment scheme for reduced representation bisulfite sequencing

Xuefeng Wang, Xiaoqing Yu, Wei Zhu, W. Richard Mccombie, Eric Antoniou, R. Scott Powers, Nicholas O. Davidson, Ellen Li, Jennie Williams

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Summary: Currently available bisulfite sequencing tools frequently suffer from low mapping rates and low methylation calls, especially for data generated from the Illumina sequencer, NextSeq. Here, we introduce a sequential trimming-and-retrieving alignment approach for investigating DNA methylation patterns, which significantly improves the number of mapped reads and covered CpG sites. The method is implemented in an automated analysis toolkit for processing bisulfite sequencing reads.

Original languageEnglish
Pages (from-to)2040-2042
Number of pages3
JournalBioinformatics
Volume31
Issue number12
DOIs
StatePublished - Jun 15 2015

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