Summary: Currently available bisulfite sequencing tools frequently suffer from low mapping rates and low methylation calls, especially for data generated from the Illumina sequencer, NextSeq. Here, we introduce a sequential trimming-and-retrieving alignment approach for investigating DNA methylation patterns, which significantly improves the number of mapped reads and covered CpG sites. The method is implemented in an automated analysis toolkit for processing bisulfite sequencing reads.

Original languageEnglish
Pages (from-to)2040-2042
Number of pages3
Issue number12
StatePublished - Jun 15 2015


Dive into the research topics of 'A trimming-and-retrieving alignment scheme for reduced representation bisulfite sequencing'. Together they form a unique fingerprint.

Cite this