TY - JOUR
T1 - A trimming-and-retrieving alignment scheme for reduced representation bisulfite sequencing
AU - Wang, Xuefeng
AU - Yu, Xiaoqing
AU - Zhu, Wei
AU - Mccombie, W. Richard
AU - Antoniou, Eric
AU - Powers, R. Scott
AU - Davidson, Nicholas O.
AU - Li, Ellen
AU - Williams, Jennie
N1 - Publisher Copyright:
© The Author 2015. Published by Oxford University Press.
PY - 2015/6/15
Y1 - 2015/6/15
N2 - Summary: Currently available bisulfite sequencing tools frequently suffer from low mapping rates and low methylation calls, especially for data generated from the Illumina sequencer, NextSeq. Here, we introduce a sequential trimming-and-retrieving alignment approach for investigating DNA methylation patterns, which significantly improves the number of mapped reads and covered CpG sites. The method is implemented in an automated analysis toolkit for processing bisulfite sequencing reads.
AB - Summary: Currently available bisulfite sequencing tools frequently suffer from low mapping rates and low methylation calls, especially for data generated from the Illumina sequencer, NextSeq. Here, we introduce a sequential trimming-and-retrieving alignment approach for investigating DNA methylation patterns, which significantly improves the number of mapped reads and covered CpG sites. The method is implemented in an automated analysis toolkit for processing bisulfite sequencing reads.
UR - http://www.scopus.com/inward/record.url?scp=84931050854&partnerID=8YFLogxK
U2 - 10.1093/bioinformatics/btv089
DO - 10.1093/bioinformatics/btv089
M3 - Article
C2 - 25681254
AN - SCOPUS:84931050854
SN - 1367-4803
VL - 31
SP - 2040
EP - 2042
JO - Bioinformatics
JF - Bioinformatics
IS - 12
ER -