TY - JOUR
T1 - A transposon-based tool for transformation and mutagenesis in trypanosomatid protozoa
AU - Damasceno, Jeziel D.
AU - Beverley, Stephen M.
AU - Tosi, Luiz R.O.
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - The ability of transposable elements to mobilize across genomes and affect the expression of genes makes them exceptional tools for genetic manipulation methodologies. Several transposon-based systems have been modified and incorporated into shuttle mutagenesis approaches in a variety of organisms. We have found that the Mos1 element, a DNA transposon from Drosophila mauritiana, is suitable and readily adaptable to a variety of strategies to the study of trypanosomatid parasitic protozoa. Trypanosomatids are the causative agents of a wide range of neglected diseases in underdeveloped regions of the globe. In this chapter we describe the basic elements and the available protocols for the in vitro use of Mos1 derivatives in the protozoan parasite Leishmania.
AB - The ability of transposable elements to mobilize across genomes and affect the expression of genes makes them exceptional tools for genetic manipulation methodologies. Several transposon-based systems have been modified and incorporated into shuttle mutagenesis approaches in a variety of organisms. We have found that the Mos1 element, a DNA transposon from Drosophila mauritiana, is suitable and readily adaptable to a variety of strategies to the study of trypanosomatid parasitic protozoa. Trypanosomatids are the causative agents of a wide range of neglected diseases in underdeveloped regions of the globe. In this chapter we describe the basic elements and the available protocols for the in vitro use of Mos1 derivatives in the protozoan parasite Leishmania.
KW - In vitro transposition
KW - Leishmania
KW - Mariner
KW - Mutagenesis
KW - Transposon
UR - http://www.scopus.com/inward/record.url?scp=84954587349&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1438-8_14
DO - 10.1007/978-1-4939-1438-8_14
M3 - Article
C2 - 25388118
AN - SCOPUS:84954587349
SN - 1064-3745
VL - 1201
SP - 235
EP - 245
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -