A transposon-based tool for transformation and mutagenesis in trypanosomatid protozoa

Jeziel D. Damasceno, Stephen M. Beverley, Luiz R.O. Tosi

Research output: Contribution to journalArticle

Abstract

The ability of transposable elements to mobilize across genomes and affect the expression of genes makes them exceptional tools for genetic manipulation methodologies. Several transposon-based systems have been modified and incorporated into shuttle mutagenesis approaches in a variety of organisms. We have found that the Mos1 element, a DNA transposon from Drosophila mauritiana, is suitable and readily adaptable to a variety of strategies to the study of trypanosomatid parasitic protozoa. Trypanosomatids are the causative agents of a wide range of neglected diseases in underdeveloped regions of the globe. In this chapter we describe the basic elements and the available protocols for the in vitro use of Mos1 derivatives in the protozoan parasite Leishmania.

Original languageEnglish
Pages (from-to)235-245
Number of pages11
JournalMethods in Molecular Biology
Volume1201
DOIs
StatePublished - Jan 1 2015

Keywords

  • In vitro transposition
  • Leishmania
  • Mariner
  • Mutagenesis
  • Transposon

Fingerprint Dive into the research topics of 'A transposon-based tool for transformation and mutagenesis in trypanosomatid protozoa'. Together they form a unique fingerprint.

  • Cite this