TY - JOUR
T1 - A transcriptomic map of murine and human alopecia areata
AU - Borcherding, Nicholas
AU - Crotts, Sydney B.
AU - Ortolan, Luana S.
AU - Henderson, Nicholas
AU - Bormann, Nicholas L.
AU - Jabbari, Ali
N1 - Publisher Copyright:
© 2020, American Society for Clinical Investigation.
PY - 2020/7/9
Y1 - 2020/7/9
N2 - Alopecia areata (AA) is a common autoimmune condition, presenting initially with loss of hair without other overt skin changes. The unremarkable appearance of the skin surface contrasts with the complex immune activity occurring at the hair follicle. AA pathogenesis is due to the loss of immune privilege of the hair follicle, leading to autoimmune attack. Although the literature has focused on CD8+ T cells, vital roles for CD4+ T cells and antigen-presenting cells have been suggested. Here, we use single-cell sequencing to reveal distinct expression profiles of immune cells in murine AA. We found clonal expansions of both CD4+ and CD8+ T cells, with shared clonotypes across varied transcriptional states. The murine AA data were used to generate highly predictive models of human AA disease. Finally, single-cell sequencing of T cells in human AA recapitulated the clonotypic findings and the gene expression of the predictive models.
AB - Alopecia areata (AA) is a common autoimmune condition, presenting initially with loss of hair without other overt skin changes. The unremarkable appearance of the skin surface contrasts with the complex immune activity occurring at the hair follicle. AA pathogenesis is due to the loss of immune privilege of the hair follicle, leading to autoimmune attack. Although the literature has focused on CD8+ T cells, vital roles for CD4+ T cells and antigen-presenting cells have been suggested. Here, we use single-cell sequencing to reveal distinct expression profiles of immune cells in murine AA. We found clonal expansions of both CD4+ and CD8+ T cells, with shared clonotypes across varied transcriptional states. The murine AA data were used to generate highly predictive models of human AA disease. Finally, single-cell sequencing of T cells in human AA recapitulated the clonotypic findings and the gene expression of the predictive models.
UR - http://www.scopus.com/inward/record.url?scp=85088209077&partnerID=8YFLogxK
U2 - 10.1172/jci.insight.137424
DO - 10.1172/jci.insight.137424
M3 - Article
C2 - 32453712
AN - SCOPUS:85088209077
SN - 2379-3708
VL - 5
JO - JCI Insight
JF - JCI Insight
IS - 13
M1 - e137424
ER -