TY - JOUR
T1 - A toolkit for a modern gynecologic oncology tissue bank
AU - Graham, Olivia
AU - Rodriguez, Jeimmy
AU - Abbott, Rachel
AU - Lomonosova, Elena
AU - Fashemi, Bisiayo
AU - Drexler, Rebecca
AU - Grither, Whitney
AU - Rodriguez, Kevin
AU - Compadre, Amanda
AU - Loeb, Megan
AU - Sanders, Brooke
AU - Kuroki, Lindsay
AU - Hagemann, Andrea R.
AU - McCourt, Carolyn
AU - Thaker, Premal H.
AU - Fuh, Katherine
AU - Powell, Matthew A.
AU - Hagemann, Ian S.
AU - Mutch, David G.
AU - Khabele, Dineo
AU - Mullen, Mary M.
N1 - Publisher Copyright:
© 2024
PY - 2024/10
Y1 - 2024/10
N2 - Objectives: Tissue banking procedures have evolved to keep pace with precision medicine, technology, emerging understanding of racial disparities, and regulatory requirements. However, there is little published guidance regarding strategies to create and maintain a successful biorepository. Our objective is to describe the infrastructure and protocols used by our Gynecologic Oncology Tissue Bank. Methods: Our Tissue Bank was founded in 1992. In August 2022, internal funding was used to modernize the Tissue Bank. We hired three full-time employees, implemented universal screening of patients treated by gynecologic oncology faculty, updated consenting protocols, and standardized communication with providers. Tumor tissue, blood derivatives, ascites, and pleural fluid were collected from eligible, consenting patients and processed. Patient-derived cell lines and organoids were generated. For quality control purposes, one formalin-fixed, paraffin-embedded (FFPE) sample per tissue site was analyzed by a board-certified pathologist. All samples were labeled and tracked in an OpenSpecimen collection protocol and clinically annotated in a secure database. Results: From August 2022 to October 2023, 227 patients (83% white, 15% Black, 1% Asian) were enrolled and 4249 specimens were collected. Adherent cell lines were generated from 15 patients with ovarian cancer and cell suspensions for organoid generation were collected from 46 patients with ovarian cancer. A recharge center was established to self-sustain the Tissue Bank. Samples have been shared with academic and commercial collaborators. Conclusions: Our Tissue Bank has enrolled a large number of diverse patients, collected numerous specimen types, and collaborated widely. The procedures described here provide guidance for other institutions establishing similar resources.
AB - Objectives: Tissue banking procedures have evolved to keep pace with precision medicine, technology, emerging understanding of racial disparities, and regulatory requirements. However, there is little published guidance regarding strategies to create and maintain a successful biorepository. Our objective is to describe the infrastructure and protocols used by our Gynecologic Oncology Tissue Bank. Methods: Our Tissue Bank was founded in 1992. In August 2022, internal funding was used to modernize the Tissue Bank. We hired three full-time employees, implemented universal screening of patients treated by gynecologic oncology faculty, updated consenting protocols, and standardized communication with providers. Tumor tissue, blood derivatives, ascites, and pleural fluid were collected from eligible, consenting patients and processed. Patient-derived cell lines and organoids were generated. For quality control purposes, one formalin-fixed, paraffin-embedded (FFPE) sample per tissue site was analyzed by a board-certified pathologist. All samples were labeled and tracked in an OpenSpecimen collection protocol and clinically annotated in a secure database. Results: From August 2022 to October 2023, 227 patients (83% white, 15% Black, 1% Asian) were enrolled and 4249 specimens were collected. Adherent cell lines were generated from 15 patients with ovarian cancer and cell suspensions for organoid generation were collected from 46 patients with ovarian cancer. A recharge center was established to self-sustain the Tissue Bank. Samples have been shared with academic and commercial collaborators. Conclusions: Our Tissue Bank has enrolled a large number of diverse patients, collected numerous specimen types, and collaborated widely. The procedures described here provide guidance for other institutions establishing similar resources.
KW - Biorepository
KW - Endometrial cancer
KW - Gynecologic oncology
KW - Ovarian cancer
KW - Tissue bank
UR - http://www.scopus.com/inward/record.url?scp=85197531126&partnerID=8YFLogxK
U2 - 10.1016/j.ygyno.2024.07.001
DO - 10.1016/j.ygyno.2024.07.001
M3 - Article
C2 - 38971004
AN - SCOPUS:85197531126
SN - 0090-8258
VL - 189
SP - 1
EP - 8
JO - Gynecologic oncology
JF - Gynecologic oncology
ER -