A tissue-culture model for the study of canine vocal fold fibroblasts

Caroline Broadley, Debra A. Gonzalez, Rhada Nair, Michael J. Koriwchak, Robert H. Ossoff, Jeffrey M. Davidson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


A tissue-culture model has been developed for the study of fibroblasts from the canine vocal fold. Laryngeal tissue (lamina propria) obtained from euthanized dogs is rinsed, cut into 1-mm3 pieces, and incubated in 5% carbon dioxide at 37° C. A confluent monolayer is established within several days. Detectable levels of elastin in the tissue culture supernatant are measured by an indirect enzyme-linked immunosorbent assay. Various external agents have been shown to affect elastin production. The effects of KTP laser irradiation, hydrocortisone (1.3 μmol/L), transforming growth factor-β (10 ng/mL), and human leukocyte elastase have been measured. Thus the canine vocal fold fibroblast tissue culture is established as a model for further investigations to improve wound healing and to understand the wound-healing process following laryngeal microsurgery.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
Issue number1
StatePublished - Jan 1995


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