A three-step reconstruction method for fluorescence molecular tomography based on compressive sensing

Yansong Zhu, Abhinav K. Jha, Jakob K. Dreyer, Hanh N.D. Le, Jin U. Kang, Per E. Roland, Dean F. Wong, Arman Rahmim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

5 Scopus citations


Fluorescence molecular tomography (FMT) is a promising tool for real time in vivo quantification of neurotransmission (NT) as we pursue in our BRAIN initiative effort. However, the acquired image data are noisy and the reconstruction problem is ill-posed. Further, while spatial sparsity of the NT effects could be exploited, traditional compressive-sensing methods cannot be directly applied as the system matrix in FMT is highly coherent. To overcome these issues, we propose and assess a three-step reconstruction method. First, truncated singular value decomposition is applied on the data to reduce matrix coherence. The resultant image data are input to a homotopy-based reconstruction strategy that exploits sparsity via ℓ1 regularization. The reconstructed image is then input to a maximum-likelihood expectation maximization (MLEM) algorithm that retains the sparseness of the input estimate and improves upon the quantitation by accurate Poisson noise modeling. The proposed reconstruction method was evaluated in a three-dimensional simulated setup with fluorescent sources in a cuboidal scattering medium with optical properties simulating human brain cortex (reduced scattering coefficient: 9.2 cm-1, absorption coefficient: 0.1 cm-1 and tomographic measurements made using pixelated detectors. In different experiments, fluorescent sources of varying size and intensity were simulated. The proposed reconstruction method provided accurate estimates of the fluorescent source intensity, with a 20% lower root mean square error on average compared to the pure-homotopy method for all considered source intensities and sizes. Further, compared with conventional ℓ2 regularized algorithm, overall, the proposed method reconstructed substantially more accurate fluorescence distribution. The proposed method shows considerable promise and will be tested using more realistic simulations and experimental setups.

Original languageEnglish
Title of host publicationOptical Tomography and Spectroscopy of Tissue XII
EditorsRobert R. Alfano, Eva Marie Sevick-Muraca, Bruce J. Tromberg, Arjun G. Yodh
ISBN (Electronic)9781510605596
StatePublished - 2017
EventOptical Tomography and Spectroscopy of Tissue XII - San Francisco, United States
Duration: Jan 30 2017Feb 1 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Tomography and Spectroscopy of Tissue XII
Country/TerritoryUnited States
CitySan Francisco


  • Compressive sensing
  • FMT
  • Noise modeling
  • Reconstruction


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