TY - JOUR
T1 - A tetravalent dengue nanoparticle stimulates antibody production in mice
AU - Silva, Elisângela F.
AU - Orsi, Mariana
AU - Andrade, Ângela L.
AU - Domingues, Rosana Z.
AU - Silva, Breno M.
AU - de Araújo, Helena R.C.
AU - Pimenta, Paulo F.P.
AU - Diamond, Michael S.
AU - Rocha, Eliseu S.O.
AU - Kroon, Erna G.
AU - Malaquias, Luiz C.C.
AU - Coelho, Luiz F.L.
N1 - Funding Information:
Financial support was provided by FAPEMIG (APQ-04684-10) and CNPq (565778/2008-0).
PY - 2012/3/22
Y1 - 2012/3/22
N2 - Background: Dengue is a major public health problem worldwide, especially in the tropical and subtropical regions of the world. Infection with a single Dengue virus (DENV) serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients experiencing secondary infection with a different serotype progresses to the severe form of the disease, dengue hemorrhagic fever/dengue shock syndrome. Currently, there are no licensed vaccines or antiviral drugs to prevent or treat dengue infections. Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines.Findings: Here, we used a murine model to evaluate the IgG production after administration of inactivated DENV corresponding to all four serotypes adsorbed to bovine serum albumin nanoparticles. This formulation induced a production of anti-DENV IgG antibodies (p < 0.001). However, plaque reduction neutralization assays with the four DENV serotypes revealed that these antibodies have no neutralizing activity in the dilutions tested.Conclusions: Our results show that while the nanoparticle system induces humoral responses against DENV, further investigation with different DENV antigens will be required to improve immunogenicity, epitope specicity, and functional activity to make this platform a viable option for DENV vaccines.
AB - Background: Dengue is a major public health problem worldwide, especially in the tropical and subtropical regions of the world. Infection with a single Dengue virus (DENV) serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients experiencing secondary infection with a different serotype progresses to the severe form of the disease, dengue hemorrhagic fever/dengue shock syndrome. Currently, there are no licensed vaccines or antiviral drugs to prevent or treat dengue infections. Biodegradable nanoparticles coated with proteins represent a promising method for in vivo delivery of vaccines.Findings: Here, we used a murine model to evaluate the IgG production after administration of inactivated DENV corresponding to all four serotypes adsorbed to bovine serum albumin nanoparticles. This formulation induced a production of anti-DENV IgG antibodies (p < 0.001). However, plaque reduction neutralization assays with the four DENV serotypes revealed that these antibodies have no neutralizing activity in the dilutions tested.Conclusions: Our results show that while the nanoparticle system induces humoral responses against DENV, further investigation with different DENV antigens will be required to improve immunogenicity, epitope specicity, and functional activity to make this platform a viable option for DENV vaccines.
KW - Humoral response
KW - Inactivated dengue vírus
KW - Nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84858716805&partnerID=8YFLogxK
U2 - 10.1186/1477-3155-10-13
DO - 10.1186/1477-3155-10-13
M3 - Article
C2 - 22439913
AN - SCOPUS:84858716805
SN - 1477-3155
VL - 10
JO - Journal of Nanobiotechnology
JF - Journal of Nanobiotechnology
M1 - 13
ER -