A Synthetic Peptide for Evaluating Protein Sequencing Capabilities: Design of ABRF-93SEQ and Results

John Rush, Philip C. Andrews, Dan L. Crimmins, Jay Gambee, Gregory A. Grant, Sheenah M. Mische, David W. Speicher

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

10 Scopus citations

Abstract

This chapter describes a synthetic peptide for evaluating protein sequencing capabilities. In a study described in the chapter, the residue most frequently assigned correctly was V3 (77 positive correct and 0 tentative correct), followed closely by A4 and A10. C5 was the most difficult residue to call correctly and was the only residue with more tentative correct than positive correct assignments. It was also the residue most frequently assigned incorrectly and was most often mistaken for S (7/24), Y (6/24), or T (3/ 24). The difficulty with tryptophan assignment contrasted sharply with serine assignment, even though these were four of the first nine residues and the two serines were present as a doublet.

Original languageEnglish
Title of host publicationTechniques in Protein Chemistry
Pages133-141
Number of pages9
EditionC
DOIs
StatePublished - Jan 1 1994

Publication series

NameTechniques in Protein Chemistry
NumberC
Volume5
ISSN (Print)1080-8914

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