TY - JOUR
T1 - A switch in distinct IκBα degradation mechanisms mediates constitutive NF-κB activation in mature B cells
AU - Fields, Erika R.
AU - Seufzer, Bradley J.
AU - Oltz, Eugene M.
AU - Miyamoto, Shigeki
PY - 2000/5/1
Y1 - 2000/5/1
N2 - Inducible activation of cytoplasmic NF-κB/Rel transcription factors occurs via proteasome-dependent degradation of an associated inhibitor, termed IκBa. Mature B lymphocytes constitutively express nuclear NF-κB, which is important for their long-term survival. The intrinsic mechanisms by which B cells constitutively activate NF-κB are unknown. In this paper we demonstrate that maintenance of NF-κB activity in primary B cells is mediated by a novel calcium-dependent, but proteasome-independent, mechanism. Moreover, we show that differentiation of conditionally transformed pre-B cells is accompanied by a switch from proteasome-dependent to proteasome- independent degradation of IκBα. Our findings indicate that IκBα degradation mechanisms are dynamic during B cell development, and ultimately establish constitutive NF-κB activity in mature B lymphocytes.
AB - Inducible activation of cytoplasmic NF-κB/Rel transcription factors occurs via proteasome-dependent degradation of an associated inhibitor, termed IκBa. Mature B lymphocytes constitutively express nuclear NF-κB, which is important for their long-term survival. The intrinsic mechanisms by which B cells constitutively activate NF-κB are unknown. In this paper we demonstrate that maintenance of NF-κB activity in primary B cells is mediated by a novel calcium-dependent, but proteasome-independent, mechanism. Moreover, we show that differentiation of conditionally transformed pre-B cells is accompanied by a switch from proteasome-dependent to proteasome- independent degradation of IκBα. Our findings indicate that IκBα degradation mechanisms are dynamic during B cell development, and ultimately establish constitutive NF-κB activity in mature B lymphocytes.
UR - http://www.scopus.com/inward/record.url?scp=0034194260&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.164.9.4762
DO - 10.4049/jimmunol.164.9.4762
M3 - Article
C2 - 10779782
AN - SCOPUS:0034194260
SN - 0022-1767
VL - 164
SP - 4762
EP - 4767
JO - Journal of Immunology
JF - Journal of Immunology
IS - 9
ER -