A single F153Sβ3 mutation causes constitutive integrin αIIbβ3 activation in a variant form of Glanzmann thrombasthenia

Sevasti B. Koukouritaki, Aye Myat M. Thinn, Katrina J. Ashworth, Juan Fang, Haley S. Slater, Lily M. Du, Huong Thi Thu Nguyen, Xavier Pillois, Alan T. Nurden, Christopher J. Ng, Jorge Di Paola, Jieqing Zhu, David A. Wilcox

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1 Scopus citations

Abstract

This report identifies a novel variant form of the inherited bleeding disorder Glanzmann thrombasthenia, exhibiting only mild bleeding in a physically active individual. The platelets cannot aggregate ex vivo with physiologic agonists of activation, although microfluidic analysis with whole blood displays moderate ex vivo platelet adhesion and aggregation consistent with mild bleeding. Immunocytometry shows reduced expression of αIIbβ3 on quiescent platelets that spontaneously bind/store fibrinogen, and activation-dependent antibodies (ligand-induced binding site–319.4 and PAC-1) report β3 extension suggesting an intrinsic activation phenotype. Genetic analysis reveals a single F153Sβ3 substitution within the βI-domain from a heterozygous T556C nucleotide substitution of ITGB3 exon 4 in conjunction with a previously reported IVS5(+1)G>A splice site mutation with undetectable platelet messenger RNA accounting for hemizygous expression of S153β3. F153 is completely conserved among β3 of several species and all human β-integrin subunits suggesting that it may play a vital role in integrin structure/function. Mutagenesis of αIIbF153Sβ3 also displays reduced levels of a constitutively activated αIIb-S153β3 on HEK293T cells. The overall structural analysis suggests that a bulky aromatic, nonpolar amino acid (F,W)153β3 is critical for maintaining the resting conformation of α2- and α1-helices of the βI-domain because small amino acid substitutions (S,A) facilitate an unhindered inward movement of the α2- and α1-helices of the βI-domain toward the constitutively active αIIbβ3 conformation, while a bulky aromatic, polar amino acid (Y) hinders such movements and restrains αIIbβ3 activation. The data collectively demonstrate that disruption of F153β3 can significantly alter normal integrin/platelet function, although reduced expression of αIIbS153β3 may be compensated by a hyperactive conformation that promotes viable hemostasis.

Original languageEnglish
Pages (from-to)3180-3191
Number of pages12
JournalBlood Advances
Volume7
Issue number13
DOIs
StatePublished - Jul 11 2023

Keywords

  • Glanzmann thrombasthenic platelets retain hemostatic function with only low-level expression of a constitutively active mutant form of αIIbF153Sβ3
  • The bulky aromatic, nonpolar structure of F153β3 amino acid is vital for normal αIIbβ3 integrin shift from resting to an activated state

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