TY - JOUR
T1 - A single-cell guide to retinal development
T2 - Cell fate decisions of multipotent retinal progenitors in scRNA-seq
AU - Shiau, Fion
AU - Ruzycki, Philip A.
AU - Clark, Brian S.
N1 - Funding Information:
We thank all members of the Clark lab for critical assessments of the manuscript and H. Halim for helpful discussion on calculating gene regulatory network energy. This work is supported by grants from the NIH (R00EY027844 to BSC ), a career development award to BSC from Research to Prevent Blindness , and an unrestricted grant to the Department of Ophthalmology and Visual Sciences from Research to Prevent Blindness in support of PAR and BSC .
Publisher Copyright:
© 2021 The Authors
PY - 2021/10
Y1 - 2021/10
N2 - Recent advances in high throughput single-cell RNA sequencing (scRNA-seq) technology have enabled the simultaneous transcriptomic profiling of thousands of individual cells in a single experiment. To investigate the intrinsic process of retinal development, researchers have leveraged this technology to quantify gene expression in retinal cells across development, in multiple species, and from numerous important models of human disease. In this review, we summarize recent applications of scRNA-seq and discuss how these datasets have complemented and advanced our understanding of retinal progenitor cell competence, cell fate specification, and differentiation. Finally, we also highlight the outstanding questions in the field that advances in single-cell data generation and analysis will soon be able to answer.
AB - Recent advances in high throughput single-cell RNA sequencing (scRNA-seq) technology have enabled the simultaneous transcriptomic profiling of thousands of individual cells in a single experiment. To investigate the intrinsic process of retinal development, researchers have leveraged this technology to quantify gene expression in retinal cells across development, in multiple species, and from numerous important models of human disease. In this review, we summarize recent applications of scRNA-seq and discuss how these datasets have complemented and advanced our understanding of retinal progenitor cell competence, cell fate specification, and differentiation. Finally, we also highlight the outstanding questions in the field that advances in single-cell data generation and analysis will soon be able to answer.
KW - Cell fate specification
KW - Competence
KW - Neurogenesis
KW - Retinal development
KW - Retinal progenitor cells
KW - Review
KW - Single-cell
KW - Single-cell rna-sequencing
KW - scRNA-seq
UR - http://www.scopus.com/inward/record.url?scp=85109209894&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2021.06.005
DO - 10.1016/j.ydbio.2021.06.005
M3 - Article
C2 - 34146533
AN - SCOPUS:85109209894
SN - 0012-1606
VL - 478
SP - 41
EP - 58
JO - Developmental Biology
JF - Developmental Biology
ER -