A single-cell atlas of murine reproductive tissues during preterm labor

Valeria Garcia-Flores, Roberto Romero, Azam Peyvandipour, Jose Galaz, Errile Pusod, Bogdan Panaitescu, Derek Miller, Yi Xu, Li Tao, Zhenjie Liu, Adi L. Tarca, Roger Pique-Regi, Nardhy Gomez-Lopez

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the syndrome of preterm labor. The best-established causal link to preterm labor is intra-amniotic infection, which involves premature activation of the parturition cascade in the reproductive tissues. Herein, we utilize single-cell RNA sequencing (scRNA-seq) to generate a single-cell atlas of the murine uterus, decidua, and cervix in a model of infection-induced preterm labor. We show that preterm labor affects the transcriptomic profiles of specific immune and non-immune cell subsets. Shared and tissue-specific gene expression signatures are identified among affected cells. Determination of intercellular communications implicates specific cell types in preterm labor-associated signaling pathways across tissues. In silico comparison of murine and human uterine cell-cell interactions reveals conserved signaling pathways implicated in labor. Thus, our scRNA-seq data provide insights into the preterm labor-driven cellular landscape and communications in reproductive tissues.

Original languageEnglish
Article number111846
JournalCell Reports
Volume42
Issue number1
DOIs
StatePublished - Jan 31 2023

Keywords

  • cervix
  • CP: Cell biology
  • CP: Molecular biology
  • decidua
  • human
  • mouse
  • scRNA-seq
  • single cell
  • uterus

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