TY - JOUR
T1 - A single affinity column step method for the purification of ricin toxin from castor beans (Ricinus communis)
AU - Simmons, Barbara M.
AU - Russell, John H.
N1 - Funding Information:
’ This investigation was supported in part by USPHS, NIH Grant CA 28533, and by Training Grant T32 GM07200, Medical Scientist Training Program, Washington University, to B.M.S.
PY - 1985/4
Y1 - 1985/4
N2 - A rapid method for purifying ricin toxin from castor beans is presented which uses a single affinity column step to obtain pure toxin from a crude extract of castor beans. A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. The selective elution of ricin toxin and agglutinin was then achieved by eluting the affinity column with a galactose gradient, which sequentially elutes the two proteins due to a difference in binding avidity to the matrix.
AB - A rapid method for purifying ricin toxin from castor beans is presented which uses a single affinity column step to obtain pure toxin from a crude extract of castor beans. A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. The selective elution of ricin toxin and agglutinin was then achieved by eluting the affinity column with a galactose gradient, which sequentially elutes the two proteins due to a difference in binding avidity to the matrix.
UR - http://www.scopus.com/inward/record.url?scp=0021990442&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90417-8
DO - 10.1016/0003-2697(85)90417-8
M3 - Article
C2 - 3993931
AN - SCOPUS:0021990442
VL - 146
SP - 206
EP - 210
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -