A single affinity column step method for the purification of ricin toxin from castor beans (Ricinus communis)

Barbara M. Simmons, John H. Russell

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A rapid method for purifying ricin toxin from castor beans is presented which uses a single affinity column step to obtain pure toxin from a crude extract of castor beans. A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. The selective elution of ricin toxin and agglutinin was then achieved by eluting the affinity column with a galactose gradient, which sequentially elutes the two proteins due to a difference in binding avidity to the matrix.

Original languageEnglish
Pages (from-to)206-210
Number of pages5
JournalAnalytical Biochemistry
Volume146
Issue number1
DOIs
StatePublished - Apr 1985

Fingerprint Dive into the research topics of 'A single affinity column step method for the purification of ricin toxin from castor beans (Ricinus communis)'. Together they form a unique fingerprint.

  • Cite this