A simplified procedure for synthesizing large quantities of highly purified uridine [β-32P]diphospho-N-acetylglucosamine

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Abstract

A simplified procedure for the synthesis of [β-32P]UDP-N-acetylglucosamine is described. This novel method utilizes commericially available enzymes, chemical acetylation, and preparative thin-layer chromatography and can be used to synthesize millicurie quantities of the sugar nucleotide at an extremely high specific activity and purity.

Original languageEnglish
Pages (from-to)264-269
Number of pages6
JournalAnalytical Biochemistry
Volume140
Issue number1
DOIs
StatePublished - Jul 1984

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