@article{c9623fe078fc42d3a9b711d253cb5747,
title = "A simple point-of-care assay accurately detects anti-spike antibodies after SARS-CoV-2 vaccination",
abstract = "Objective: Lateral flow assays (LFA) are sensitive for detecting antibodies to SARS-CoV-2 proteins within weeks after infection. This study tested samples from immunocompetent adults, and those receiving treatments for chronic inflammatory diseases (CID), before and after mRNA SARS-CoV-2 vaccination. Methods: We compared results obtained with the COVIBLOCK Covid-19 LFA to those obtained by anti-spike (S) ELISA. Results: The LFA detected anti-S antibodies in 29 of 29 (100%) of the immunocompetent and 110 of 126 (87.3%) of the CID participants after vaccination. Semiquantitative LFA scores were statistically significantly lower in samples from immunosuppressed participants, and were significantly correlated with anti-S antibody levels measured by ELISA. Conclusions: This simple LFA test is a practical alternative to laboratory-based assays for detecting anti-S antibodies after infection or vaccination. This type of test may be most useful for testing people in outpatient or resource-limited settings.",
keywords = "Antibody test, Immunocompromise, SARS-COV-2, Vaccination",
author = "Greene, {Sarah E.} and Yuefang Huang and Wooseob Kim and Liebeskind, {Mariel J.} and Vinay Chandrasekaran and Zhuoming Liu and Parakkal Deepak and Paley, {Michael A.} and Daphne Lew and Monica Yang and Mehrdad Matloubian and Gensler, {Lianne S.} and Nakamura, {Mary C.} and O'Hallaran, {Jane A.} and Presti, {Rachel M.} and Whelan, {Sean P.J.} and Buchser, {William J.} and Kim, {Alfred H.J.} and Weil, {Gary J.}",
note = "Funding Information: This work was financially supported by the Foundation for Barnes-Jewish Hospital ( https://www.foundationbarnesjewish.org/ ) [grant 4618 ] to [GJW], and National Institute of Health Training of the Pediatric Physician Scientist [grant T32 HD043010 supported SEG]. COVaRiPAD was funded/supported by The Leona M. and Harry B. Helmsley Charitable Trust, Washington University Digestive Disease Research Core Center ( NIDDK P30DK052574 ), Washington University Rheumatic Diseases Research Resource-Based Center ( NIAMS P30AR073752 ), The Judy Miniace Research Fund for the Washington University Lupus Clinic, NIAID Collaborative Influenza Vaccine Innovation Centers contract 75N93019C00051 for the Washington University Infectious Disease Clinical Research Unit COVID sample collection, and UCSF investigators were funded by a Seeding Bold Ideas Grant from the Marcus Program in Precision Medicine Innovation, PREMIER , a NIAMS P30 Center ( P30AR070155 ) and the Russell/Engleman Rheumatology Research Center. WU368 study was supported by the Barnes-Jewish Hospital Foundation, the Siteman Cancer Center grant P30CA091842 from the National Cancer Institute of the National Institutes of Health, and the Washington University Institute of Clinical and Translational Sciences grant UL1TR002345 from the National Center for Advancing Translational Sciences of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
month = feb,
doi = "10.1016/j.jcvp.2023.100135",
language = "English",
volume = "3",
journal = "Journal of Clinical Virology Plus",
issn = "2667-0380",
number = "1",
}