A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

Sau W. Cheung, James P. Crane, Ann Johnson, Lyn Simms, Joan Reid

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin‐EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalPrenatal Diagnosis
Volume7
Issue number6
DOIs
StatePublished - Jul 1987

Keywords

  • Amniotic fluid cell culture
  • Prenatal diagnosis
  • Prometaphase chromosomes

Fingerprint Dive into the research topics of 'A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures'. Together they form a unique fingerprint.

  • Cite this