A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

Sau W. Cheung; James P. Crane; Ann Johnson; Lyn Simms; Joan Reid

doi:10.1002/pd.1970070602

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

Sau W. Cheung, James P. Crane, Ann Johnson, Lyn Simms, Joan Reid

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7 Scopus citations

Abstract

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin‐EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.

Original language	English
Pages (from-to)	383-388
Number of pages	6
Journal	Prenatal Diagnosis
Volume	7
Issue number	6
DOIs	https://doi.org/10.1002/pd.1970070602
State	Published - Jul 1987

Keywords

Amniotic fluid cell culture
Prenatal diagnosis
Prometaphase chromosomes

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10.1002/pd.1970070602

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abstract = "A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin‐EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.",

keywords = "Amniotic fluid cell culture, Prenatal diagnosis, Prometaphase chromosomes",

author = "Cheung, {Sau W.} and Crane, {James P.} and Ann Johnson and Lyn Simms and Joan Reid",

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AU - Cheung, Sau W.

AU - Crane, James P.

AU - Johnson, Ann

AU - Simms, Lyn

AU - Reid, Joan

PY - 1987/7

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AB - A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures is described. The technique is based upon several key steps including: (1) reduced colcemid concentration, (2) reduced exposure to trypsin‐EDTA, and (3) maintaining cells in single suspension by adjusting cell concentration appropriately. Chromosomes with banding resolution up ot 800 bands per haploid set can be routinely produced. The described methodology is particularly useful in defining and establishing the clinical significance of subtle structural aberrations.

KW - Amniotic fluid cell culture

KW - Prenatal diagnosis

KW - Prometaphase chromosomes

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ER -

A simple method for preparing prometaphase chromosomes from amniotic fluid cell cultures

Abstract

Keywords

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