A simple method for hemoglobin measurement in cell culture system

B. S. Baliga, K. Isoyama, G. Longmore, Y. M. Yang, A. K. Shah, V. N. Mankad

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A simple method of hemoglobin analysis in a cell culture system is described. Hemoglobins synthesized in cell cultures are labeled with radioactive amino acids. The cell extract containing radiolabeled hemoglobin is mixed with A, F, S, C, hemoglobin markers and separated by cellulose acetate electrophoresis. Individual bands of hemoglobin are cut from the gel and analyzed for radioactivity. This method is especially useful for determination of newly synthesized minute amount of hemoglobin in cell extracts that are difficult to visualize by staining procedure.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalBlood Cells
Volume18
Issue number2
StatePublished - 1992

Keywords

  • burst-forming unit-erythroid
  • hemoglobin electrophoresis
  • hemoglobin estimation
  • K
  • mouse erythroleukemic cell line

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