A sequence at the inside end of IS50 down regulates transposition

Karen W. Dodson, Douglas E. Berg

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalPlasmid
Volume25
Issue number2
DOIs
StatePublished - Mar 1991

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