A sequence at the inside end of IS50 down regulates transposition

Karen W. Dodson; Douglas E. Berg

doi:10.1016/0147-619X(91)90028-U

A sequence at the inside end of IS50 down regulates transposition

Karen W. Dodson, Douglas E. Berg

Department of Molecular Microbiology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.

Original language	English
Pages (from-to)	145-148
Number of pages	4
Journal	Plasmid
Volume	25
Issue number	2
DOIs	https://doi.org/10.1016/0147-619X(91)90028-U
State	Published - Mar 1991

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title = "A sequence at the inside end of IS50 down regulates transposition",

abstract = "Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.",

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T1 - A sequence at the inside end of IS50 down regulates transposition

AU - Dodson, Karen W.

AU - Berg, Douglas E.

PY - 1991/3

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N2 - Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.

AB - Deletion analysis has shown that the segment at the IS50 inside (I) end that is needed for efficient transposition is ~19 bp long. Dam methylation at two 5′ GATC sequences within this segment decreases I-end transposition activity. A third 5′ GATC sequence is present at bp 21-24 of the I end. The comparisons presented here show that extension of the I end from 19 to 24 bp decreases its transposition activity in dam cells 5- to 50-fold, depending on the overall transposon structure.

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U2 - 10.1016/0147-619X(91)90028-U

DO - 10.1016/0147-619X(91)90028-U

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AN - SCOPUS:0025808657

VL - 25

SP - 145

EP - 148

JO - Plasmid

JF - Plasmid

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IS - 2

ER -

A sequence at the inside end of IS50 down regulates transposition

Abstract

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