TY - JOUR
T1 - A sensitive and specific method for the measurement of monophosphoinositide at a microregional level in brain
AU - Cicero, T. J.
AU - Sherman, W. R.
N1 - Funding Information:
‘This research was supported in part by USPHS grants MH20717 and NS05159. T. J. C. is recipient of Research Scientist Development Award I-KZ-MH-70180 and W. R. S. of Research Career Program Award GM-21,863. The mass spectrometer was purchased by Health Sciences Advancement Award 5S04PR06115.
PY - 1973/7
Y1 - 1973/7
N2 - A method is described wherein monophosphoinositide is quantitatively extracted from as little as 1 mg of brain, deacylated to glycerophosphoryl inositol, trimethylsilylated and gas chromatographed. Amounts of glycerophosphoryl inositol as small as 5 ng (15 pmoles) have been chromatographed. The flame ionization detector response is linear from this level to 10 μg. This method gives values for monophosphoinositide in excellent agreement with those obtained with much larger tissue samples using the previously available techniques. Other advantages of the gas chromatographic method are its high degree of specificity, due to the increased resolution of the separation technique, and the considerable increase in the speed with which multiple determinations can be carried out.
AB - A method is described wherein monophosphoinositide is quantitatively extracted from as little as 1 mg of brain, deacylated to glycerophosphoryl inositol, trimethylsilylated and gas chromatographed. Amounts of glycerophosphoryl inositol as small as 5 ng (15 pmoles) have been chromatographed. The flame ionization detector response is linear from this level to 10 μg. This method gives values for monophosphoinositide in excellent agreement with those obtained with much larger tissue samples using the previously available techniques. Other advantages of the gas chromatographic method are its high degree of specificity, due to the increased resolution of the separation technique, and the considerable increase in the speed with which multiple determinations can be carried out.
UR - http://www.scopus.com/inward/record.url?scp=0015794152&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(73)90243-1
DO - 10.1016/0003-2697(73)90243-1
M3 - Article
C2 - 4353534
AN - SCOPUS:0015794152
SN - 0003-2697
VL - 54
SP - 32
EP - 39
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -