A selective inhibitor of the sperm-specific potassium channel SLO3 impairs human sperm function

Maximilian Lyon, Ping Li, Juan J. Ferreira, Roman M. Lazarenko, Sujay V. Kharade, Meghan Kramer, Samantha J. McClenahan, Emily Days, Joshua A. Bauer, Brittany D. Spitznagel, C. David Weaver, Aluet Borrego Alvarez, Lis C. Puga Molina, Pascale Lybaert, Saayli Khambekar, Alicia Liu, Craig W. Lindsley, Jerod Denton, Celia M. Santi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

To fertilize an oocyte, the membrane potential of both mouse and human sperm must hyperpolarize (become more negative inside). Determining the molecular mechanisms underlying this hyperpolarization is vital for developing new contraceptive methods and detecting causes of idiopathic male infertility. In mouse sperm, hyperpolarization is caused by activation of the sperm-specific potassium (K+) channel SLO3 [C. M. Santi et al., FEBS Lett. 584, 1041–1046 (2010)]. In human sperm, it has long been unclear whether hyperpolarization depends on SLO3 or the ubiquitous K+ channel SLO1 [N. Mannowetz, N. M. Naidoo, S. A. S. Choo, J. F. Smith, P. V. Lishko, Elife 2, e01009 (2013), C. Brenker et al., Elife 3, e01438 (2014), and S. A. Mansell, S. J. Publicover, C. L. R. Barratt, S. M. Wilson, Mol. Hum. Reprod. 20, 392–408 (2014)]. In this work, we identified the first selective inhibitor for human SLO3—VU0546110—and showed that it completely blocked heterologous SLO3 currents and endogenous K+ currents in human sperm. This compound also prevented sperm from hyperpolarizing and undergoing hyperactivated motility and induced acrosome reaction, which are necessary to fertilize an egg. We conclude that SLO3 is the sole K+ channel responsible for hyperpolarization and significantly contributes to the fertilizing ability of human sperm. Moreover, SLO3 is a good candidate for contraceptive development, and mutation of this gene is a possible cause of idiopathic male infertility.

Original languageEnglish
Article numbere2212338120
JournalProceedings of the National Academy of Sciences of the United States of America
Volume120
Issue number4
DOIs
StatePublished - Jan 24 2023

Keywords

  • SLO3
  • capacitation
  • drug discovery
  • human sperm
  • ion channels

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