A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

Jasmin Bahr; Gereon Poschmann; Andreas Jungmann; Martin Busch; Zhaoping Ding; Jens Vogt; Ria Zalfen; Julia Steinhausen; Arlen Aurora Euan Martínez; Thorsten Wachtmeister; Daniel Rickert; Tobias Lautwein; Christina Alter; Junedh M. Amrute; Kory J. Lavine; Karl Köhrer; Bodo Levkau; Patrick Most; Kai Stühler; Julia Hesse; Jürgen Schrader

doi:10.1038/s42003-025-08083-y

A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

Jasmin Bahr, Gereon Poschmann, Andreas Jungmann, Martin Busch, Zhaoping Ding, Jens Vogt, Ria Zalfen, Julia Steinhausen, Arlen Aurora Euan Martínez, Thorsten Wachtmeister, Daniel Rickert, Tobias Lautwein, Christina Alter, Junedh M. Amrute, Kory J. Lavine, Karl Köhrer, Bodo Levkau, Patrick Most, Kai Stühler, Julia HesseJürgen Schrader

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Abstract

Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.

Original language	English
Article number	675
Journal	Communications Biology
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s42003-025-08083-y
State	Published - Dec 2025

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Bahr, J., Poschmann, G., Jungmann, A., Busch, M., Ding, Z., Vogt, J., Zalfen, R., Steinhausen, J., Euan Martínez, A. A., Wachtmeister, T., Rickert, D., Lautwein, T., Alter, C., Amrute, J. M., Lavine, K. J., Köhrer, K., Levkau, B., Most, P., Stühler, K., ... Schrader, J. (2025). A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts. Communications Biology, 8(1), Article 675. https://doi.org/10.1038/s42003-025-08083-y

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title = "A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts",

abstract = "Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.",

author = "Jasmin Bahr and Gereon Poschmann and Andreas Jungmann and Martin Busch and Zhaoping Ding and Jens Vogt and Ria Zalfen and Julia Steinhausen and {Euan Mart{\'i}nez}, {Arlen Aurora} and Thorsten Wachtmeister and Daniel Rickert and Tobias Lautwein and Christina Alter and Amrute, {Junedh M.} and Lavine, {Kory J.} and Karl K{\"o}hrer and Bodo Levkau and Patrick Most and Kai St{\"u}hler and Julia Hesse and J{\"u}rgen Schrader",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",

year = "2025",

month = dec,

doi = "10.1038/s42003-025-08083-y",

language = "English",

volume = "8",

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Bahr, J, Poschmann, G, Jungmann, A, Busch, M, Ding, Z, Vogt, J, Zalfen, R, Steinhausen, J, Euan Martínez, AA, Wachtmeister, T, Rickert, D, Lautwein, T, Alter, C, Amrute, JM, Lavine, KJ, Köhrer, K, Levkau, B, Most, P, Stühler, K, Hesse, J & Schrader, J 2025, 'A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts', Communications Biology, vol. 8, no. 1, 675. https://doi.org/10.1038/s42003-025-08083-y

TY - JOUR

T1 - A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

AU - Bahr, Jasmin

AU - Poschmann, Gereon

AU - Jungmann, Andreas

AU - Busch, Martin

AU - Ding, Zhaoping

AU - Vogt, Jens

AU - Zalfen, Ria

AU - Steinhausen, Julia

AU - Euan Martínez, Arlen Aurora

AU - Wachtmeister, Thorsten

AU - Rickert, Daniel

AU - Lautwein, Tobias

AU - Alter, Christina

AU - Amrute, Junedh M.

AU - Lavine, Kory J.

AU - Köhrer, Karl

AU - Levkau, Bodo

AU - Most, Patrick

AU - Stühler, Kai

AU - Hesse, Julia

AU - Schrader, Jürgen

PY - 2025/12

Y1 - 2025/12

N2 - Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.

AB - Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of protein secretion. Specific in-vivo labeling of miCF proteins via biotin ligase TurboID using the POSTN promotor mirrors the in-vitro data. In summary, we identify numerous paracrine factors specifically secreted from CF in mice and humans. This secretome atlas may lead to new biomarkers and/or therapeutic targets for the activated CF.

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U2 - 10.1038/s42003-025-08083-y

DO - 10.1038/s42003-025-08083-y

M3 - Article

C2 - 40301568

AN - SCOPUS:105003884073

SN - 2399-3642

VL - 8

JO - Communications Biology

JF - Communications Biology

IS - 1

M1 - 675

ER -

A secretome atlas of cardiac fibroblasts from healthy and infarcted mouse hearts

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