A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii

S. Taylor, A. Barragan, C. Su, B. Fux, S. J. Fentress, K. Tang, W. L. Beatty, H. El Hajj, M. Jerome, M. S. Behnke, M. White, J. C. Wootton, L. D. Sibley

Research output: Contribution to journalArticle

362 Scopus citations

Abstract

Toxoplasma gondii strains differ dramatically in virulence despite being genetically very similar. Genetic mapping revealed two closely adjacent quantitative trait loci on parasite chromosome VIIa that control the extreme virulence of the type I lineage. Positional cloning identified the candidate virulence gene ROP18, a highly polymorphic serine-threonine kinase that was secreted into the host cell during parasite invasion. Transfection of the virulent ROP18 allele into a nonpathogenic type III strain increased growth and enhanced mortality by 4 to 5 logs. These attributes of ROP18 required kinase activity, which revealed that secretion of effectors is a major component of parasite virulence.

Original languageEnglish
Pages (from-to)1776-1780
Number of pages5
JournalScience
Volume314
Issue number5806
DOIs
StatePublished - Dec 15 2006

Fingerprint Dive into the research topics of 'A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii'. Together they form a unique fingerprint.

  • Cite this

    Taylor, S., Barragan, A., Su, C., Fux, B., Fentress, S. J., Tang, K., Beatty, W. L., El Hajj, H., Jerome, M., Behnke, M. S., White, M., Wootton, J. C., & Sibley, L. D. (2006). A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science, 314(5806), 1776-1780. https://doi.org/10.1126/science.1133643