TY - JOUR
T1 - A sandwich enzyme-linked immunoadsorbent assay for detection of murine macrophage colony-stimulating factor (CSF-1)
AU - Lokeshwar, Balakrishna L.
AU - Lin, Hsiu san
PY - 1989/9/29
Y1 - 1989/9/29
N2 - A simple three-layer sandwich enzyme-linked immunoadsorbent assay (sandwich-ELISA) has been developed for murine macrophage colony-stimulating factor-1 (CSF-1) using the two monoclonal antibodies on which we recently reported (J. Immunol. (1988) 141, 483). The anti-CSF-1 monoclonal antibodies used in this assay recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1. This assay is specific to murine CSF-1. Recombinant human macrophage colony-stimulating factor, murine GM-CSF, or IL-3, either alone or together with CSF-1, does not interfere with the assay. The advantage of this assay over other reported immunoassays for CSF-1 is that radiolabeled or large quantities of purified CSF-1 are not required. This sandwich-ELISA compares favorbly with other assays in its rapidity, simplicity, and sensitivity.
AB - A simple three-layer sandwich enzyme-linked immunoadsorbent assay (sandwich-ELISA) has been developed for murine macrophage colony-stimulating factor-1 (CSF-1) using the two monoclonal antibodies on which we recently reported (J. Immunol. (1988) 141, 483). The anti-CSF-1 monoclonal antibodies used in this assay recognize different epitopes of the same antigen, thereby permitting the detection of low amounts of CSF-1. This assay is specific to murine CSF-1. Recombinant human macrophage colony-stimulating factor, murine GM-CSF, or IL-3, either alone or together with CSF-1, does not interfere with the assay. The advantage of this assay over other reported immunoassays for CSF-1 is that radiolabeled or large quantities of purified CSF-1 are not required. This sandwich-ELISA compares favorbly with other assays in its rapidity, simplicity, and sensitivity.
KW - Colony-stimulating factor 1
KW - In vitro colony assay
KW - Monoclonal antibody, rat
KW - Sandwich-ELISA
UR - http://www.scopus.com/inward/record.url?scp=0024396837&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(89)90036-7
DO - 10.1016/0022-1759(89)90036-7
M3 - Article
C2 - 2677146
AN - SCOPUS:0024396837
SN - 0022-1759
VL - 123
SP - 123
EP - 129
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -