TY - JOUR
T1 - A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control
AU - Odom, Audrey R.
AU - Stahlberg, Alke
AU - Wente, S. R.
AU - York, John D.
PY - 2000/3/17
Y1 - 2000/3/17
N2 - Phospholipase C and two inositol polyphosphate (IP) kinases constitute a signaling pathway that regulates nuclear messenger RNA export through production of inositol hexakisphosphate (IP6). The inositol 1,4,5- trisphosphate kinase of this pathway in Saccharomyces cerevisiae, designated Ipk2, was found to be identical to Arg82, a regulator of the transcriptional complex ArgR-Mcm1. Synthesis of inositol 1,4,5,6-tetrakisphosphate, but not IP6, was required for gene regulation through ArgR-Mcm1. Thus, the phospholipase C pathway produces multiple IP messengers that modulate distinct nuclear processes. The results reveal a direct mechanism by which activation of IP signaling may control gene expression.
AB - Phospholipase C and two inositol polyphosphate (IP) kinases constitute a signaling pathway that regulates nuclear messenger RNA export through production of inositol hexakisphosphate (IP6). The inositol 1,4,5- trisphosphate kinase of this pathway in Saccharomyces cerevisiae, designated Ipk2, was found to be identical to Arg82, a regulator of the transcriptional complex ArgR-Mcm1. Synthesis of inositol 1,4,5,6-tetrakisphosphate, but not IP6, was required for gene regulation through ArgR-Mcm1. Thus, the phospholipase C pathway produces multiple IP messengers that modulate distinct nuclear processes. The results reveal a direct mechanism by which activation of IP signaling may control gene expression.
UR - http://www.scopus.com/inward/record.url?scp=0034677903&partnerID=8YFLogxK
U2 - 10.1126/science.287.5460.2026
DO - 10.1126/science.287.5460.2026
M3 - Article
C2 - 10720331
AN - SCOPUS:0034677903
SN - 0036-8075
VL - 287
SP - 2026
EP - 2029
JO - Science
JF - Science
IS - 5460
ER -