TY - GEN
T1 - A robust statistical estimation (RoSE) algorithm jointly recovers the 3D location and intensity of single molecules accurately and precisely
AU - Mazidi, Hesam
AU - Nehorai, Arye
AU - Lew, Matthew D.
N1 - Funding Information:
Research reported in this publication was supported by the National Science Foundation under grant number ECCS-1653777 and by the National Institute of General Medical Sciences of the National Institutes of Health under grant number R35GM124858.
Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
PY - 2018
Y1 - 2018
N2 - In single-molecule (SM) super-resolution microscopy, the complexity of a biological structure, high molecular density, and a low signal-to-background ratio (SBR) may lead to imaging artifacts without a robust localization algorithm. Moreover, engineered point spread functions (PSFs) for 3D imaging pose difficulties due to their intricate features. We develop a Robust Statistical Estimation algorithm, called RoSE, that enables joint estimation of the 3D location and photon counts of SMs accurately and precisely using various PSFs under conditions of high molecular density and low SBR.
AB - In single-molecule (SM) super-resolution microscopy, the complexity of a biological structure, high molecular density, and a low signal-to-background ratio (SBR) may lead to imaging artifacts without a robust localization algorithm. Moreover, engineered point spread functions (PSFs) for 3D imaging pose difficulties due to their intricate features. We develop a Robust Statistical Estimation algorithm, called RoSE, that enables joint estimation of the 3D location and photon counts of SMs accurately and precisely using various PSFs under conditions of high molecular density and low SBR.
KW - 3D super-resolution fluorescence microscopy
KW - joint location and brightness estimation
KW - joint sparsity
KW - multi-dimensional reconstruction algorithm
KW - single-molecule localization microscopy
UR - http://www.scopus.com/inward/record.url?scp=85047414764&partnerID=8YFLogxK
U2 - 10.1117/12.2289994
DO - 10.1117/12.2289994
M3 - Conference contribution
AN - SCOPUS:85047414764
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Single Molecule Spectroscopy and Superresolution Imaging XI
A2 - Koberling, Felix
A2 - Gregor, Ingo
A2 - Enderlein, Jorg
A2 - Erdmann, Rainer
A2 - Gryczynski, Zygmunt Karol
PB - SPIE
T2 - Single Molecule Spectroscopy and Superresolution Imaging XI 2018
Y2 - 27 January 2018 through 28 January 2018
ER -