TY - JOUR
T1 - A rat liver protein with potential thrombin activity
T2 - Properties and partial purification
AU - Shah, D. V.
AU - Suttie, J. W.
AU - Grant, G. A.
N1 - Funding Information:
1 Research support,ed by t.he College of Agricultural and Life Sciences, Universit,y of Wisconsin-Madison, in part by (irant No. AM-14881 from the Nnt,ional Tnstitntes of Health, U. S. Public Healt,h Service, and in part, hy a National Institutes of Healt,h Training (irant No. (:>I-002Xi-BCH.
PY - 1973/11
Y1 - 1973/11
N2 - A protein which generates thrombin activity when treated with the venom from Echis carinatus has been shown to build up in the liver microsomes of vitamin K-deficient or anticoagulant-treated rats. The amount of this protein decreases rapidly when vitamin K is given to these animals. The protein has been partially purified. It differs from prothrombin in its ability to adsorb to BaSO4, its electrophoretic mobility, and chromatography on DEAE-cellulose. It does, however, react with antibody prepared against rat prothrombin, and it apparently contains the thrombin portion of prothrombin. These observations are consistent with the hypothesis that this protein represents a liver precursor protein which is converted to plasma prothrombin in a vitamin K-dependent step.
AB - A protein which generates thrombin activity when treated with the venom from Echis carinatus has been shown to build up in the liver microsomes of vitamin K-deficient or anticoagulant-treated rats. The amount of this protein decreases rapidly when vitamin K is given to these animals. The protein has been partially purified. It differs from prothrombin in its ability to adsorb to BaSO4, its electrophoretic mobility, and chromatography on DEAE-cellulose. It does, however, react with antibody prepared against rat prothrombin, and it apparently contains the thrombin portion of prothrombin. These observations are consistent with the hypothesis that this protein represents a liver precursor protein which is converted to plasma prothrombin in a vitamin K-dependent step.
UR - http://www.scopus.com/inward/record.url?scp=0015812645&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(73)90478-5
DO - 10.1016/0003-9861(73)90478-5
M3 - Article
C2 - 4206203
AN - SCOPUS:0015812645
SN - 0003-9861
VL - 159
SP - 483
EP - 491
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -