TY - JOUR
T1 - A rapid method for chromatographic analysis of oligosaccharides on Bio-Gel P-4
AU - Natowicz, Marvin
AU - Baenziger, Jacques U.
N1 - Funding Information:
1 This study was supported by NIH Grant No. ROl CA21923 and NSF Grant PCM-7911486. M.N. is a Medical Scientist Trainee supported by Grant GM07200 from the National Institute of General Medical Sciences. This study was also supported by the following companies: Brown & Williamson Tobacco Corporation: Larus and Brother Company, Inc.; Liggett & Myers, Inc.; Lorillard, a Division of Loews Theatres, Inc.; Philip Morris, Inc.; R. J. Reynolds Tobacco Company; United States Tobacco Company; and Tobacco Associates, Inc.
PY - 1980/6
Y1 - 1980/6
N2 - We have developed a method for the rapid analysis of oligosaccharides and their degradation products by chromatographic separation on Bio-Gel P-4 (-400 mesh). Flow rates of 64 ml cm-2/h-1 can be attained at 55-100 psi with either a single column of 0.6 × 100 cm or two coupled columns each 0.6 × 100 cm, permitting analyses to be carried out in 1.5 or 3.0 h, respectively. Differences of 1 glucose equivalent can be precisely distinguished for oligomers at least as large as 15 glucose equivalents. The applicability of this method for the analysis of glycoprotein-derived oligosaccharides is demonstrated. By assigning a value of 1 glucose equivalent per neutral sugar residue and 2 glucose equivalents per amino sugar residue in such oligosaccharides, the effect of various exo- and endoglycosidase digestions can be rapidly and precisely monitored. These columns are extremely stable, providing continuous operation for at least 9 months with over 300 sample applications without deterioration.
AB - We have developed a method for the rapid analysis of oligosaccharides and their degradation products by chromatographic separation on Bio-Gel P-4 (-400 mesh). Flow rates of 64 ml cm-2/h-1 can be attained at 55-100 psi with either a single column of 0.6 × 100 cm or two coupled columns each 0.6 × 100 cm, permitting analyses to be carried out in 1.5 or 3.0 h, respectively. Differences of 1 glucose equivalent can be precisely distinguished for oligomers at least as large as 15 glucose equivalents. The applicability of this method for the analysis of glycoprotein-derived oligosaccharides is demonstrated. By assigning a value of 1 glucose equivalent per neutral sugar residue and 2 glucose equivalents per amino sugar residue in such oligosaccharides, the effect of various exo- and endoglycosidase digestions can be rapidly and precisely monitored. These columns are extremely stable, providing continuous operation for at least 9 months with over 300 sample applications without deterioration.
UR - https://www.scopus.com/pages/publications/0019198690
U2 - 10.1016/0003-2697(80)90439-X
DO - 10.1016/0003-2697(80)90439-X
M3 - Article
C2 - 7446983
AN - SCOPUS:0019198690
SN - 0003-2697
VL - 105
SP - 159
EP - 164
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -