A rapid and sensitive radioenzymatic assay for catechol estrogens in tissues

Steven M. Paul, Julius Axelrod

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A rapid and sensitive radioenzymatic assay for measuring catechol estrogens in tissue has been developed. This assay is based on converting the relatively labile catechol estrogens to stable O-methylated derivatives by the enzyme catechol-O-methyl-transferase. Using a radioactive methyl donor of high specific activity (3H-S-adenosylmethionine) and solvent extraction with non-polar solvents a sensitivity of 25 pg can be achieved. The specificity of this assay was confirmed by thin layer chromatography and mass spectral analysis of the reaction products. Catechol estrogens in rat liver are significantly decreased after ovariectomy and markedly increased by treatment with estrogen.

Original languageEnglish
Pages (from-to)493-502
Number of pages10
JournalLife Sciences
Volume21
Issue number4
DOIs
StatePublished - Aug 15 1977
Externally publishedYes

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