A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

Yiming Zhang; Brian J. DeBosch

doi:10.1016/j.xpro.2022.101489

A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

Yiming Zhang
, Brian J. DeBosch

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).

Original language	English
Article number	101489
Journal	STAR Protocols
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2022.101489
State	Published - Sep 16 2022

Keywords

Metabolism
Molecular Biology

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10.1016/j.xpro.2022.101489

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title = "A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase",

abstract = "Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).",

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N2 - Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).

AB - Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).

KW - Metabolism

KW - Molecular Biology

UR - https://www.scopus.com/pages/publications/85132890629

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DO - 10.1016/j.xpro.2022.101489

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ER -

A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

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Keywords

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