@article{a5d21f27e93c4c2482b82bdcd9b3a3cb,
title = "A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase",
abstract = "Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).",
keywords = "Metabolism, Molecular Biology",
author = "Yiming Zhang and DeBosch, {Brian J.}",
note = "Funding Information: Part of this study was funded by a sponsored research agreement awarded by Polaris Pharmaceuticals (to B.J.D.). B.J.D. is the lead inventor on US Patent Application #17/050,318. Relevant US Patent Publication #US2021/0077598, toward which the presented data are material. Funding Information: This work was supported by grants from the NIDDK ( 1R01DK126622-01A1 , B.J.D.), NHLBI ( 1R01HL147968-01A1 , B.J.D.), AASLD (Pilot Research Award, B.J.D.), NCCIH ( 1R21AT010520-01 , B.J.D.), NIH / National Center for Advancing Translational Sciences (NCATS, #UL1TR002345 , B.J.D.), NIDDK ( R56DK115764 , B.J.D.), AGA-Gilead Sciences Research Scholar Award in Liver Disease (B.J.D.), the AGA-Allergan Foundation Pilot Research Award in Non-Alcoholic Fatty Liver Disease (B.J.D.), the Washington University Digestive Disease Research Core Center ( P30DK052574 ), Washington University Diabetes Research Center ( P30DK020579 ), the Nutrition & Obesity Research Center ( P30DK056341 ), the Association for Aging Research Junior Faculty Award (B.J.D.), the Robert Wood Johnson Foundation (B.J.D.), the Washington University Center for Autophagy Therapeutics Research (B.J.D.), and the Longer Life Foundation (B.J.D.). Y.Z. is a pre-doctoral fellow supported by the Washington University School of Medicine Pediatric Gastroenterology Research Training Grant ( T32DK077653 ), and the Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship ( F31DK131875 ). ADI-PEG 20 was a kind gift from Dr. John S. Bomalaski (Polaris Pharmaceuticals, Inc., San Diego, CA, USA). Funding Information: This work was supported by grants from the NIDDK (1R01DK126622-01A1, B.J.D.), NHLBI (1R01HL147968-01A1, B.J.D.), AASLD (Pilot Research Award, B.J.D.), NCCIH (1R21AT010520-01, B.J.D.), NIH/National Center for Advancing Translational Sciences (NCATS, #UL1TR002345, B.J.D.), NIDDK (R56DK115764, B.J.D.), AGA-Gilead Sciences Research Scholar Award in Liver Disease (B.J.D.), the AGA-Allergan Foundation Pilot Research Award in Non-Alcoholic Fatty Liver Disease (B.J.D.), the Washington University Digestive Disease Research Core Center (P30DK052574), Washington University Diabetes Research Center (P30DK020579), the Nutrition & Obesity Research Center (P30DK056341), the Association for Aging Research Junior Faculty Award (B.J.D.), the Robert Wood Johnson Foundation (B.J.D.), the Washington University Center for Autophagy Therapeutics Research (B.J.D.), and the Longer Life Foundation (B.J.D.). Y.Z. is a pre-doctoral fellow supported by the Washington University School of Medicine Pediatric Gastroenterology Research Training Grant (T32DK077653), and the Ruth L. Kirschstein National Research Service Award (NRSA) Individual Predoctoral Fellowship (F31DK131875). ADI-PEG 20 was a kind gift from Dr. John S. Bomalaski (Polaris Pharmaceuticals, Inc. San Diego, CA, USA). Conceptualization, Y.Z. and B.J.D.; Methodology, Y.Z.; Investigation, Y.Z.; Data Curation, Y.Z.; Formal Analysis, Y.Z.; Visualization, Y.Z.; Pictures, Y.Z.; G.A. Graphics, Y.Z.; Writing – Original Draft, Y.Z.; Writing – Review and Editing, Y.Z. and B.J.D.; Supervision, B.J.D.; Project Administration, Y.Z. and B.J.D.; and Funding Acquisition, Y.Z. and B.J.D. Part of this study was funded by a sponsored research agreement awarded by Polaris Pharmaceuticals (to B.J.D.). B.J.D. is the lead inventor on US Patent Application #17/050,318. Relevant US Patent Publication #US2021/0077598, toward which the presented data are material. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = sep,
day = "16",
doi = "10.1016/j.xpro.2022.101489",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
number = "3",
}