@inbook{0ee23bd4942c4e3694f897376c0301f9,
title = "A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing",
abstract = "Untargeted metabolomics has rapidly become a profiling method of choice in many areas of research, including mitochondrial biology. Most commonly, untargeted metabolomics is performed with liquid chromatography/mass spectrometry because it enables measurement of a relatively wide range of physiochemically diverse molecules. Specifically, to assess energy pathways that are associated with mitochondrial metabolism, hydrophilic interaction liquid chromatography (HILIC) is often applied before analysis with a high-resolution accurate mass instrument. The workflow produces large, complex data files that are impractical to analyze manually. Here, we present a protocol to perform untargeted metabolomics on biofluids such as plasma, urine, and cerebral spinal fluid with a HILIC separation and an Orbitrap mass spectrometer. Our protocol describes each step of the analysis in detail, from preparation of solvents for chromatography to selecting parameters during data processing.",
keywords = "Accurate mass, Data-dependent acquisition, HILIC, High-resolution, Liquid chromatography, Mass spectrometry, Metabolites, Metabolomics, Profiling, Quality assurance, Quality control",
author = "Souza, {Amanda L.} and Patti, {Gary J.}",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1266-8_27",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "357--382",
booktitle = "Methods in Molecular Biology",
}