A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice

Lila S. Nolan; Qingqing Gong; Heather N. Hofmeister; Misty Good

doi:10.1016/j.xpro.2021.100951

A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice

Lila S. Nolan, Qingqing Gong, Heather N. Hofmeister, Misty Good

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).

Original language	English
Article number	100951
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100951
State	Published - Dec 17 2021

Keywords

Developmental biology
Health Sciences
Microbiology
Model Organisms
Molecular Biology

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abstract = "Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).",

keywords = "Developmental biology, Health Sciences, Microbiology, Model Organisms, Molecular Biology",

author = "Nolan, {Lila S.} and Qingqing Gong and Hofmeister, {Heather N.} and Misty Good",

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AB - Recapitulating human NEC using animal models has been insightful in dissecting the signaling pathways, immune-mediated mechanisms, genetic signatures, and the intestinal architecture of NEC. This protocol describes an in vivo murine NEC model, using hypoxia and formula containing lipopolysaccharide and enteric bacteria derived from an infant with NEC. With this mouse model, we aim to further dissect NEC pathogenesis and develop new therapeutic strategies. For complete details on the use and execution of this protocol, please refer to Mihi et al. (2021).

KW - Developmental biology

KW - Health Sciences

KW - Microbiology

KW - Model Organisms

KW - Molecular Biology

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JO - STAR Protocols

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ER -

A protocol for the induction of experimental necrotizing enterocolitis in neonatal mice

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