A proposed route to independent measurements of tight junction conductance at discrete cell junctions

Lushan Zhou, Yuhan Zeng, Lane A. Baker, Jianghui Hou

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Direct recording of tight junction permeability is of pivotal importance to many biologic fields. Previous approaches bear an intrinsic disadvantage due to the difficulty of separating tight junction conductance from nearby membrane conductance. Here, we propose the design of Double whole-cell Voltage Clamp - Ion Conductance Microscopy (DVC-ICM) based on previously demonstrated potentiometric scanning of local conductive pathways. As proposed, DVC-ICM utilizes two coordinated whole-cell patch-clamps to neutralize the apical membrane current during potentiometric scanning, which in models described here will profoundly enhance the specificity of tight junction recording. Several potential pitfalls are considered, evaluated and addressed with alternative countermeasures.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalTissue Barriers
Volume3
Issue number4
DOIs
StatePublished - Oct 2 2015

Keywords

  • SICM
  • conductance
  • ion channel
  • patch clamp
  • tight junction

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