A practical process for the preparation of [32P]S1P and binding assay for S1P receptor ligands

Adam J. Rosenberg, Hui Liu, Zhude Tu

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Sphingosine-1-phosphate receptors (S1PRs) are important regulators of vascular permeability, inflammation, angiogenesis and vascular maturation. Identifying a specific S1PR PET radioligand is imperative, but it is hindered by the complexity and variability of current for binding affinity measurement procedures. Herein, we report a streamlined protocol for radiosynthesis of [32P]S1P with good radiochemical yield (36-50%) and high radiochemical purity (>99%). We also report a reproducible procedure for determining the binding affinity for compounds targeting S1PRs in vitro.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalApplied Radiation and Isotopes
Volume102
DOIs
StatePublished - Aug 1 2015

Keywords

  • Competitive binding assay
  • Phosphorus-32
  • Radioligand
  • Sphingosine 1-phosphate
  • Sphingosine 1-phosphate Receptors
  • Sphingosine kinase 1

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