TY - JOUR
T1 - A practical process for the preparation of [32P]S1P and binding assay for S1P receptor ligands
AU - Rosenberg, Adam J.
AU - Liu, Hui
AU - Tu, Zhude
N1 - Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Sphingosine-1-phosphate receptors (S1PRs) are important regulators of vascular permeability, inflammation, angiogenesis and vascular maturation. Identifying a specific S1PR PET radioligand is imperative, but it is hindered by the complexity and variability of current for binding affinity measurement procedures. Herein, we report a streamlined protocol for radiosynthesis of [32P]S1P with good radiochemical yield (36-50%) and high radiochemical purity (>99%). We also report a reproducible procedure for determining the binding affinity for compounds targeting S1PRs in vitro.
AB - Sphingosine-1-phosphate receptors (S1PRs) are important regulators of vascular permeability, inflammation, angiogenesis and vascular maturation. Identifying a specific S1PR PET radioligand is imperative, but it is hindered by the complexity and variability of current for binding affinity measurement procedures. Herein, we report a streamlined protocol for radiosynthesis of [32P]S1P with good radiochemical yield (36-50%) and high radiochemical purity (>99%). We also report a reproducible procedure for determining the binding affinity for compounds targeting S1PRs in vitro.
KW - Competitive binding assay
KW - Phosphorus-32
KW - Radioligand
KW - Sphingosine 1-phosphate
KW - Sphingosine 1-phosphate Receptors
KW - Sphingosine kinase 1
UR - http://www.scopus.com/inward/record.url?scp=84928580233&partnerID=8YFLogxK
U2 - 10.1016/j.apradiso.2015.04.010
DO - 10.1016/j.apradiso.2015.04.010
M3 - Article
C2 - 25931137
AN - SCOPUS:84928580233
SN - 0969-8043
VL - 102
SP - 5
EP - 9
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
ER -