TY - JOUR
T1 - A POU domain transcription factor-dependent program regulates axon pathfinding in the vertebrate visual system
AU - Erkman, Linda
AU - Yates, Paul A.
AU - McLaughlin, Todd
AU - McEvilly, Robert J.
AU - Whisenhunt, Thomas
AU - O'Connell, Shawn M.
AU - Krones, Anna I.
AU - Kirby, Michael A.
AU - Rapaport, David H.
AU - Bermingham, John R.
AU - O'Leary, Dennis D.M.
AU - Rosenfeld, Michael G.
N1 - Funding Information:
We gratefully acknowledge Rebecca Tuttle, Aimee Ryan, and Bogi Andersen for many helpful discussions and critical reading of the manuscript; Jeremy Dasen, Yasushi Nakagawa, Alex Langston, and Tod Sugihara for sharing reagents; Mary Frazer for expert and dedicated help with animal care; Thomas Herman for technical assistance with sequencing; and Peggy Myer for help with illustrations. Our special thanks go to Dr. Geneviève Rougon (CNRS, France) for the generous gift of 26W sensory cell line. M. G. R. is an investigator with HHMI; R. J. M. is a recipient of NARSAD young investigator award. This work was supported by grant #17-97-2-7016 NMTB to M. A. K.; DAMD to M. G. R.; and NIH grants to D. H. R. (EY11875), D. D. M. O. (EY07025), and M. G. R. (NS34934).
PY - 2000
Y1 - 2000
N2 - Axon pathfinding relies on the ability of the growth cone to detect and interpret guidance cues and to modulate cytoskeletal changes in response to these signals. We report that the murine POU domain transcription factor Brn-3.2 regulates pathfinding in retinal ganglion cell (RGC) axons at multiple points along their pathways and the establishment of topographic order in the superior colliculus. Using representational difference analysis, we identified Brn-3.2 gene targets likely to act on axon guidance at the levels of transcription, cell-cell interaction, and signal transduction, including the actin-binding LIM domain protein abLIM. We present evidence that abLIM plays a crucial role in RGC axon pathfinding, sharing functional similarity with its C. elegans homolog, UNC-115. Our findings provide insights into a Brn-3.2-directed hierarchical program linking signaling events to cytoskeletal changes required for axon pathfinding.
AB - Axon pathfinding relies on the ability of the growth cone to detect and interpret guidance cues and to modulate cytoskeletal changes in response to these signals. We report that the murine POU domain transcription factor Brn-3.2 regulates pathfinding in retinal ganglion cell (RGC) axons at multiple points along their pathways and the establishment of topographic order in the superior colliculus. Using representational difference analysis, we identified Brn-3.2 gene targets likely to act on axon guidance at the levels of transcription, cell-cell interaction, and signal transduction, including the actin-binding LIM domain protein abLIM. We present evidence that abLIM plays a crucial role in RGC axon pathfinding, sharing functional similarity with its C. elegans homolog, UNC-115. Our findings provide insights into a Brn-3.2-directed hierarchical program linking signaling events to cytoskeletal changes required for axon pathfinding.
UR - http://www.scopus.com/inward/record.url?scp=17744391748&partnerID=8YFLogxK
U2 - 10.1016/S0896-6273(00)00153-7
DO - 10.1016/S0896-6273(00)00153-7
M3 - Article
C2 - 11163266
AN - SCOPUS:17744391748
SN - 0896-6273
VL - 28
SP - 779
EP - 792
JO - Neuron
JF - Neuron
IS - 3
ER -