A potently neutralizing antibody protects mice against SARS-CoV-2 infection

Wafaa B. Alsoussi, Jackson S. Turner, James B. Case, Haiyan Zhao, Aaron J. Schmitz, Julian Q. Zhou, Rita E. Chen, Tingting Lei, Amena A. Rizk, Katherine M. McIntire, Emma S. Winkler, Julie M. Fox, Natasha M. Kafai, Larissa B. Thackray, Ahmed O. Hassan, Fatima Amanat, Florian Krammer, Corey T. Watson, Steven H. Kleinstein, Daved H. FremontMichael S. Diamond, Ali H. Ellebedy

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for millions of infections and hundreds of thousands of deaths globally. There are no widely available licensed therapeutics against SARS-CoV-2, highlighting an urgent need for effective interventions. The virus enters host cells through binding of a receptor-binding domain within its trimeric spike glycoprotein to human angiotensin-converting enzyme 2. In this article, we describe the generation and characterization of a panel of murine mAbs directed against the receptor-binding domain. One mAb, 2B04, neutralized wild-type SARS-CoV-2 in vitro with remarkable potency (half-maximal inhibitory concentration of <2 ng/ml). In a murine model of SARS-CoV-2 infection, 2B04 protected challenged animals from weight loss, reduced lung viral load, and blocked systemic dissemination. Thus, 2B04 is a promising candidate for an effective antiviral that can be used to prevent SARS-CoV-2 infection.

Original languageEnglish
Pages (from-to)915-922
Number of pages8
JournalJournal of Immunology
Volume205
Issue number4
DOIs
StatePublished - Aug 15 2020

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    Alsoussi, W. B., Turner, J. S., Case, J. B., Zhao, H., Schmitz, A. J., Zhou, J. Q., Chen, R. E., Lei, T., Rizk, A. A., McIntire, K. M., Winkler, E. S., Fox, J. M., Kafai, N. M., Thackray, L. B., Hassan, A. O., Amanat, F., Krammer, F., Watson, C. T., Kleinstein, S. H., ... Ellebedy, A. H. (2020). A potently neutralizing antibody protects mice against SARS-CoV-2 infection. Journal of Immunology, 205(4), 915-922. https://doi.org/10.4049/jimmunol.2000583