TY - JOUR
T1 - A pilot study of galunisertib plus stereotactic body radiotherapy in patients with advanced hepatocellular carcinoma
AU - Reiss, Kim A.
AU - Wattenberg, Max M.
AU - Damjanov, Nevena
AU - Dunphy, Elizabeth Prechtel
AU - Jacobs-Small, Mona
AU - Lubas, M. Judy
AU - Robinson, James
AU - Dicicco, Lisa
AU - Garcia-Marcano, Luis
AU - Giannone, Michael A.
AU - Karasic, Thomas B.
AU - Furth, Emma E.
AU - Carpenter, Erica L.
AU - Wojcieszynski, Andrzej P.
AU - Vonderheide, Robert H.
AU - Beatty, Gregory L.
AU - Ben-Josef, Edgar
N1 - Publisher Copyright:
© 2020 American Association for Cancer Research.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - TGFb is a pleiotropic cytokine with immunosuppressive activity. In preclinical models, blockade of TGFb enhances the activity of radiation and invokes T-cell antitumor immunity. Here, we combined galunisertib, an oral TGFb inhibitor, with stereotactic body radiotherapy (SBRT) in patients with advanced hepatocellular carcinoma (HCC) and assessed safety, efficacy, and immunologic correlatives. Patients (n ¼ 15) with advanced HCC who progressed on, were intolerant of, or refused sorafenib were treated with galunisertib (150 mg orally twice a day) on days 1 to 14 of each 28-day cycle. A single dose of SBRT (18-Gy) was delivered between days 15 to 28 of cycle 1. Site of index lesions treated with SBRT included liver (9 patients), lymph node (4 patients), and lung (2 patients). Blood for high-dimensional single cell profiling was collected. The most common treatment-related adverse events were fatigue (53%), abdominal pain (46.6%), nausea (40%), and increased alkaline phosphatase (40%). There were two instances of grade 2 alkaline phosphatase increase and two instances of grade 2 bilirubin increase. One patient developed grade 3 achalasia, possibly related to treatment. Two patients achieved a partial response. Treatment with galunisertib was associated with a decrease in the frequency of activated T regulatory cells in the blood. Distinct peripheral blood leukocyte populations detected at baseline distinguished progressors from nonprogressors. Nonprogressors also had increased CD8þPD-1þTIGITþ T cells in the blood after treatment. We found galunisertib combined with SBRT to be well tolerated and associated with antitumor activity in patients with HCC. Pre- and posttreatment immune profiling of the blood was able to distinguish patients with progression versus nonprogression.
AB - TGFb is a pleiotropic cytokine with immunosuppressive activity. In preclinical models, blockade of TGFb enhances the activity of radiation and invokes T-cell antitumor immunity. Here, we combined galunisertib, an oral TGFb inhibitor, with stereotactic body radiotherapy (SBRT) in patients with advanced hepatocellular carcinoma (HCC) and assessed safety, efficacy, and immunologic correlatives. Patients (n ¼ 15) with advanced HCC who progressed on, were intolerant of, or refused sorafenib were treated with galunisertib (150 mg orally twice a day) on days 1 to 14 of each 28-day cycle. A single dose of SBRT (18-Gy) was delivered between days 15 to 28 of cycle 1. Site of index lesions treated with SBRT included liver (9 patients), lymph node (4 patients), and lung (2 patients). Blood for high-dimensional single cell profiling was collected. The most common treatment-related adverse events were fatigue (53%), abdominal pain (46.6%), nausea (40%), and increased alkaline phosphatase (40%). There were two instances of grade 2 alkaline phosphatase increase and two instances of grade 2 bilirubin increase. One patient developed grade 3 achalasia, possibly related to treatment. Two patients achieved a partial response. Treatment with galunisertib was associated with a decrease in the frequency of activated T regulatory cells in the blood. Distinct peripheral blood leukocyte populations detected at baseline distinguished progressors from nonprogressors. Nonprogressors also had increased CD8þPD-1þTIGITþ T cells in the blood after treatment. We found galunisertib combined with SBRT to be well tolerated and associated with antitumor activity in patients with HCC. Pre- and posttreatment immune profiling of the blood was able to distinguish patients with progression versus nonprogression.
UR - http://www.scopus.com/inward/record.url?scp=85100457892&partnerID=8YFLogxK
U2 - 10.1158/1535-7163.MCT-20-0632
DO - 10.1158/1535-7163.MCT-20-0632
M3 - Article
C2 - 33268571
AN - SCOPUS:85100457892
SN - 1535-7163
VL - 20
SP - 389
EP - 397
JO - Molecular Cancer Therapeutics
JF - Molecular Cancer Therapeutics
IS - 2
ER -