A physical map of the mouse genome

Simon G. Gregory; Mandeep Sekhon; Jacqueline Schein; Shaying Zhao; Kazutoyo Osoegawa; Carol E. Scott; Richard S. Evans; Paul W. Burridge; Tony V. Cox; Christopher A. Fox; Richard D. Hutton; Ian R. Mulienger; Kimbly J. Phillips; James Smith; Jim Stalker; Glen J. Threadgold; Ewan Birney; Kristine Wylie; Asif Chinwalia; John Wallis; La Deana Hillier; Jason Carter; Tony Galge; Sara Jaeger; Colin Kremitzki; Dan Layman; Jason Maas; Rebecca McGrane; Kelly Mead; Rebecca Walker; Steven Jones; Michael Smith; Jennifer Asano; Ian Bosdet; Susanna Chan; Suganthi Chittaranjan; Readman Chiu; Chris Fjeil; Dan Fuhrmann; Noreen Girn; Catharine Gray; Ran Guin; Letticia Hsiao; Martin Krzywinski; Reta Kutsche; Soo Sen Lee; Carrie Mathewson; Candice McLeavy; Steven Messervier; Steven Ness; Pawan Pandoh; Anna Liisa Prabhu; Parvaneh Saeedi; Duane Smallus; Lorraine Spence; Jeff Stott; Sheryl Taylor; Wesley Terpstra; Miranda Tsai; Jill Vardy; Natasja Wye; George Yang; Sofiya Shatsman; Bola Ayodeji; Kelta Geer; Getahun Tsegaye; Alla Shvartsbeyn; Elizabeth Gebregeorgis; Margaret Kroi; Daniel Russell; Larry Overton; Joel A. Malek; Mike Holmes; Michael Heaney; Jyoti Shetty; Tamara Feldblyum; William C. Nierman; Joseph J. Catanese; Tim Hubbard; Robert H. Waterston; Jane Rogers; Pieter J. De Jong; Claire M. Fraser; John D. McPherson; David R. Bentley

doi:10.1038/nature00957

A physical map of the mouse genome

Simon G. Gregory, Mandeep Sekhon, Jacqueline Schein, Shaying Zhao, Kazutoyo Osoegawa, Carol E. Scott, Richard S. Evans, Paul W. Burridge, Tony V. Cox, Christopher A. Fox, Richard D. Hutton, Ian R. Mulienger, Kimbly J. Phillips, James Smith, Jim Stalker, Glen J. Threadgold, Ewan Birney, Kristine Wylie, Asif Chinwalia, John WallisLa Deana Hillier, Jason Carter, Tony Galge, Sara Jaeger, Colin Kremitzki, Dan Layman, Jason Maas, Rebecca McGrane, Kelly Mead, Rebecca Walker, Steven Jones, Michael Smith, Jennifer Asano, Ian Bosdet, Susanna Chan, Suganthi Chittaranjan, Readman Chiu, Chris Fjeil, Dan Fuhrmann, Noreen Girn, Catharine Gray, Ran Guin, Letticia Hsiao, Martin Krzywinski, Reta Kutsche, Soo Sen Lee, Carrie Mathewson, Candice McLeavy, Steven Messervier, Steven Ness, Pawan Pandoh, Anna Liisa Prabhu, Parvaneh Saeedi, Duane Smallus, Lorraine Spence, Jeff Stott, Sheryl Taylor, Wesley Terpstra, Miranda Tsai, Jill Vardy, Natasja Wye, George Yang, Sofiya Shatsman, Bola Ayodeji, Kelta Geer, Getahun Tsegaye, Alla Shvartsbeyn, Elizabeth Gebregeorgis, Margaret Kroi, Daniel Russell, Larry Overton, Joel A. Malek, Mike Holmes, Michael Heaney, Jyoti Shetty, Tamara Feldblyum, William C. Nierman, Joseph J. Catanese, Tim Hubbard, Robert H. Waterston, Jane Rogers, Pieter J. De Jong, Claire M. Fraser, John D. McPherson, David R. Bentley

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Abstract

A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16, 992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51, 486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.

Original language	English
Pages (from-to)	743-750
Number of pages	8
Journal	Nature
Volume	418
Issue number	6899
DOIs	https://doi.org/10.1038/nature00957
State	Published - Aug 15 2002

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Gregory, S. G., Sekhon, M., Schein, J., Zhao, S., Osoegawa, K., Scott, C. E., Evans, R. S., Burridge, P. W., Cox, T. V., Fox, C. A., Hutton, R. D., Mulienger, I. R., Phillips, K. J., Smith, J., Stalker, J., Threadgold, G. J., Birney, E., Wylie, K., Chinwalia, A., ... Bentley, D. R. (2002). A physical map of the mouse genome. Nature, 418(6899), 743-750. https://doi.org/10.1038/nature00957

@article{cc86565735d546c19118039ff578fbdf,

title = "A physical map of the mouse genome",

abstract = "A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16, 992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51, 486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.",

author = "Gregory, {Simon G.} and Mandeep Sekhon and Jacqueline Schein and Shaying Zhao and Kazutoyo Osoegawa and Scott, {Carol E.} and Evans, {Richard S.} and Burridge, {Paul W.} and Cox, {Tony V.} and Fox, {Christopher A.} and Hutton, {Richard D.} and Mulienger, {Ian R.} and Phillips, {Kimbly J.} and James Smith and Jim Stalker and Threadgold, {Glen J.} and Ewan Birney and Kristine Wylie and Asif Chinwalia and John Wallis and Hillier, {La Deana} and Jason Carter and Tony Galge and Sara Jaeger and Colin Kremitzki and Dan Layman and Jason Maas and Rebecca McGrane and Kelly Mead and Rebecca Walker and Steven Jones and Michael Smith and Jennifer Asano and Ian Bosdet and Susanna Chan and Suganthi Chittaranjan and Readman Chiu and Chris Fjeil and Dan Fuhrmann and Noreen Girn and Catharine Gray and Ran Guin and Letticia Hsiao and Martin Krzywinski and Reta Kutsche and Lee, {Soo Sen} and Carrie Mathewson and Candice McLeavy and Steven Messervier and Steven Ness and Pawan Pandoh and Prabhu, {Anna Liisa} and Parvaneh Saeedi and Duane Smallus and Lorraine Spence and Jeff Stott and Sheryl Taylor and Wesley Terpstra and Miranda Tsai and Jill Vardy and Natasja Wye and George Yang and Sofiya Shatsman and Bola Ayodeji and Kelta Geer and Getahun Tsegaye and Alla Shvartsbeyn and Elizabeth Gebregeorgis and Margaret Kroi and Daniel Russell and Larry Overton and Malek, {Joel A.} and Mike Holmes and Michael Heaney and Jyoti Shetty and Tamara Feldblyum and Nierman, {William C.} and Catanese, {Joseph J.} and Tim Hubbard and Waterston, {Robert H.} and Jane Rogers and {De Jong}, {Pieter J.} and Fraser, {Claire M.} and McPherson, {John D.} and Bentley, {David R.}",

note = "Funding Information: The authors acknowledge the support of the Wellcome Trust, the National Institutes of Health and the US Department of Energy. We are grateful to the web team at the Sanger Institute for assistance with developing map displays, to P. Deloukas for RH map analysis, and E. Arnold-Berkowits, S. Lo, J. Gill and all present and past members of the Institute for Genomic Research BAC end sequencing team for the sequencing work.",

year = "2002",

month = aug,

day = "15",

doi = "10.1038/nature00957",

language = "English",

volume = "418",

pages = "743--750",

journal = "Nature",

issn = "0028-0836",

number = "6899",

}

Gregory, SG, Sekhon, M, Schein, J, Zhao, S, Osoegawa, K, Scott, CE, Evans, RS, Burridge, PW, Cox, TV, Fox, CA, Hutton, RD, Mulienger, IR, Phillips, KJ, Smith, J, Stalker, J, Threadgold, GJ, Birney, E, Wylie, K, Chinwalia, A, Wallis, J, Hillier, LD, Carter, J, Galge, T, Jaeger, S, Kremitzki, C, Layman, D, Maas, J, McGrane, R, Mead, K, Walker, R, Jones, S, Smith, M, Asano, J, Bosdet, I, Chan, S, Chittaranjan, S, Chiu, R, Fjeil, C, Fuhrmann, D, Girn, N, Gray, C, Guin, R, Hsiao, L, Krzywinski, M, Kutsche, R, Lee, SS, Mathewson, C, McLeavy, C, Messervier, S, Ness, S, Pandoh, P, Prabhu, AL, Saeedi, P, Smallus, D, Spence, L, Stott, J, Taylor, S, Terpstra, W, Tsai, M, Vardy, J, Wye, N, Yang, G, Shatsman, S, Ayodeji, B, Geer, K, Tsegaye, G, Shvartsbeyn, A, Gebregeorgis, E, Kroi, M, Russell, D, Overton, L, Malek, JA, Holmes, M, Heaney, M, Shetty, J, Feldblyum, T, Nierman, WC, Catanese, JJ, Hubbard, T, Waterston, RH, Rogers, J, De Jong, PJ, Fraser, CM, McPherson, JD & Bentley, DR 2002, 'A physical map of the mouse genome', Nature, vol. 418, no. 6899, pp. 743-750. https://doi.org/10.1038/nature00957

TY - JOUR

T1 - A physical map of the mouse genome

AU - Gregory, Simon G.

AU - Sekhon, Mandeep

AU - Schein, Jacqueline

AU - Zhao, Shaying

AU - Osoegawa, Kazutoyo

AU - Scott, Carol E.

AU - Evans, Richard S.

AU - Burridge, Paul W.

AU - Cox, Tony V.

AU - Fox, Christopher A.

AU - Hutton, Richard D.

AU - Mulienger, Ian R.

AU - Phillips, Kimbly J.

AU - Smith, James

AU - Stalker, Jim

AU - Threadgold, Glen J.

AU - Birney, Ewan

AU - Wylie, Kristine

AU - Chinwalia, Asif

AU - Wallis, John

AU - Hillier, La Deana

AU - Carter, Jason

AU - Galge, Tony

AU - Jaeger, Sara

AU - Kremitzki, Colin

AU - Layman, Dan

AU - Maas, Jason

AU - McGrane, Rebecca

AU - Mead, Kelly

AU - Walker, Rebecca

AU - Jones, Steven

AU - Smith, Michael

AU - Asano, Jennifer

AU - Bosdet, Ian

AU - Chan, Susanna

AU - Chittaranjan, Suganthi

AU - Chiu, Readman

AU - Fjeil, Chris

AU - Fuhrmann, Dan

AU - Girn, Noreen

AU - Gray, Catharine

AU - Guin, Ran

AU - Hsiao, Letticia

AU - Krzywinski, Martin

AU - Kutsche, Reta

AU - Lee, Soo Sen

AU - Mathewson, Carrie

AU - McLeavy, Candice

AU - Messervier, Steven

AU - Ness, Steven

AU - Pandoh, Pawan

AU - Prabhu, Anna Liisa

AU - Saeedi, Parvaneh

AU - Smallus, Duane

AU - Spence, Lorraine

AU - Stott, Jeff

AU - Taylor, Sheryl

AU - Terpstra, Wesley

AU - Tsai, Miranda

AU - Vardy, Jill

AU - Wye, Natasja

AU - Yang, George

AU - Shatsman, Sofiya

AU - Ayodeji, Bola

AU - Geer, Kelta

AU - Tsegaye, Getahun

AU - Shvartsbeyn, Alla

AU - Gebregeorgis, Elizabeth

AU - Kroi, Margaret

AU - Russell, Daniel

AU - Overton, Larry

AU - Malek, Joel A.

AU - Holmes, Mike

AU - Heaney, Michael

AU - Shetty, Jyoti

AU - Feldblyum, Tamara

AU - Nierman, William C.

AU - Catanese, Joseph J.

AU - Hubbard, Tim

AU - Waterston, Robert H.

AU - Rogers, Jane

AU - De Jong, Pieter J.

AU - Fraser, Claire M.

AU - McPherson, John D.

AU - Bentley, David R.

N1 - Funding Information: The authors acknowledge the support of the Wellcome Trust, the National Institutes of Health and the US Department of Energy. We are grateful to the web team at the Sanger Institute for assistance with developing map displays, to P. Deloukas for RH map analysis, and E. Arnold-Berkowits, S. Lo, J. Gill and all present and past members of the Institute for Genomic Research BAC end sequencing team for the sequencing work.

PY - 2002/8/15

Y1 - 2002/8/15

N2 - A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16, 992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51, 486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.

AB - A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16, 992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51, 486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.

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U2 - 10.1038/nature00957

DO - 10.1038/nature00957

M3 - Article

C2 - 12181558

AN - SCOPUS:0037102417

SN - 0028-0836

VL - 418

SP - 743

EP - 750

JO - Nature

JF - Nature

IS - 6899

ER -

A physical map of the mouse genome

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