A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export

John D. York; Audrey R. Odom; Robert Murphy; Eric B. Ives; Susan R. Wente

doi:10.1126/science.285.5424.96

A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export

John D. York, Audrey R. Odom, Robert Murphy, Eric B. Ives, Susan R. Wente

Division of Infectious Disease

Research output: Contribution to journal › Article › peer-review

454 Scopus citations

Abstract

In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC). The three genes identified encode phospholipase C and two potential inositol polyphosphate kinases. Together, these constitute a signaling pathway from phosphatidylinositol 4,5-bisphosphate to inositol hexakisphosphate (IP₆). The common downstream effects of mutations in each component were deficiencies in IP₆ synthesis and messenger RNA export, indicating a role for IP₆ in GLE1 function and messenger RNA export.

Original language	English
Pages (from-to)	96-100
Number of pages	5
Journal	Science
Volume	285
Issue number	5424
DOIs	https://doi.org/10.1126/science.285.5424.96
State	Published - Jul 2 1999

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abstract = "In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC). The three genes identified encode phospholipase C and two potential inositol polyphosphate kinases. Together, these constitute a signaling pathway from phosphatidylinositol 4,5-bisphosphate to inositol hexakisphosphate (IP6). The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export.",

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AU - Ives, Eric B.

AU - Wente, Susan R.

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AB - In order to identify additional factors required for nuclear export of messenger RNA, a genetic screen was conducted with a yeast mutant deficient in a factor Gle1p, which associates with the nuclear pore complex (NPC). The three genes identified encode phospholipase C and two potential inositol polyphosphate kinases. Together, these constitute a signaling pathway from phosphatidylinositol 4,5-bisphosphate to inositol hexakisphosphate (IP6). The common downstream effects of mutations in each component were deficiencies in IP6 synthesis and messenger RNA export, indicating a role for IP6 in GLE1 function and messenger RNA export.

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A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export

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