@article{eb35091d22a0406680e870e7737e56ad,
title = "A pdf neuropeptide gene mutation and ablation of PDF neurons each cause severe abnormalities of behavioral circadian rhythms in Drosophila",
abstract = "The mechanisms by which circadian pacemaker systems transmit timing information to control behavior are largely unknown. Here, we define two critical features of that mechanism in Drosophila. We first describe animals mutant for the pdf neuropeptide gene, which is expressed by most of the candidate pacemakers (LN(v) neurons). Next, we describe animals in which pdf neurons were selectively ablated. Both sets of animals produced similar behavioral phenotypes. Both sets entrained to light, but both were largely arrhythmic under constant conditions. A minority of each pdf variant exhibited weak to moderate free-running rhythmicity. These results confirm the assignment of LN(v) neurons as the principal circadian pacemakers controlling daily locomotion in Drosophila. They also implicate PDF as the principal circadian transmitter.",
author = "Renn, {Susan C.P.} and Park, {Jae H.} and Michael Rosbash and Hall, {Jeffrey C.} and Taghert, {Paul H.}",
note = "Funding Information: This work was supported by NIH grant GM-33205 (J. H. P., M. R., and J. C. H.), by NIMH NRSA fellowship F32MH-1196 (J. H. P.), and by NIH grant NS-21749 and NSF grant IBN-973003 (P. H. T.). We thank Hermann Steller, Bruce Hay, and the Bloomington Drosophila Stock Center for fly strains. We are grateful to Gail Fasciani and Pamela Jacobs for assisting with behavioral tests, Mei Han for immunohistochemistry, Gyunghee Lee for helping with confocal microscopy, Edward Dougherty for photographic assistance. We thank Monika Stengl for discussions and appreciate comments on the manuscript from Joel Levine and Michael Menaker. ",
year = "1999",
month = dec,
day = "23",
doi = "10.1016/S0092-8674(00)81676-1",
language = "English",
volume = "99",
pages = "791--802",
journal = "Cell",
issn = "0092-8674",
number = "7",
}