Abstract
A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in raw milk following enrichment.
| Original language | English |
|---|---|
| Pages (from-to) | 1801-1804 |
| Number of pages | 4 |
| Journal | Journal of clinical microbiology |
| Volume | 36 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1998 |
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