TY - JOUR
T1 - A one precursor one siRNA model for pol IV-dependent siRNA biogenesis
AU - Zhai, Jixian
AU - Bischof, Sylvain
AU - Wang, Haifeng
AU - Feng, Suhua
AU - Lee, Tzuu Fen
AU - Teng, Chong
AU - Chen, Xinyuan
AU - Park, Soo Young
AU - Liu, Linshan
AU - Gallego-Bartolome, Javier
AU - Liu, Wanlu
AU - Henderson, Ian R.
AU - Meyers, Blake C.
AU - Ausin, Israel
AU - Jacobsen, Steven E.
N1 - Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/10/8
Y1 - 2015/10/8
N2 - RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs (siRNAs) that target DNA methylation. Here, we demonstrate that Pol IV-dependent RNAs (P4RNAs) from wild-type Arabidopsis are surprisingly short in length (30 to 40 nt) and mirror 24-nt siRNAs in distribution, abundance, strand bias, and 5′-adenine preference. P4RNAs exhibit transcription start sites similar to Pol II products and are featured with 5′-monophosphates and 3′-misincorporated nucleotides. The 3′-misincorporation preferentially occurs at methylated cytosines on the template DNA strand, suggesting a co-transcriptional feedback to siRNA biogenesis by DNA methylation to reinforce silencing locally. These results highlight an unusual mechanism of Pol IV transcription and suggest a "one precursor, one siRNA" model for the biogenesis of 24-nt siRNAs in Arabidopsis.
AB - RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs (siRNAs) that target DNA methylation. Here, we demonstrate that Pol IV-dependent RNAs (P4RNAs) from wild-type Arabidopsis are surprisingly short in length (30 to 40 nt) and mirror 24-nt siRNAs in distribution, abundance, strand bias, and 5′-adenine preference. P4RNAs exhibit transcription start sites similar to Pol II products and are featured with 5′-monophosphates and 3′-misincorporated nucleotides. The 3′-misincorporation preferentially occurs at methylated cytosines on the template DNA strand, suggesting a co-transcriptional feedback to siRNA biogenesis by DNA methylation to reinforce silencing locally. These results highlight an unusual mechanism of Pol IV transcription and suggest a "one precursor, one siRNA" model for the biogenesis of 24-nt siRNAs in Arabidopsis.
UR - https://www.scopus.com/pages/publications/84943786479
U2 - 10.1016/j.cell.2015.09.032
DO - 10.1016/j.cell.2015.09.032
M3 - Article
C2 - 26451488
AN - SCOPUS:84943786479
SN - 0092-8674
VL - 163
SP - 445
EP - 455
JO - Cell
JF - Cell
IS - 2
ER -