A novel system for mitigation of ectopic transgene expression induced by adenoviral vectors

P. N. Reynolds, M. D. Holmes, Y. Adachi, L. Kaliberova, D. T. Curiel

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Adenoviral (Ad) vectors are good candidates for gene therapy in view of their high in vivo gene delivery efficiency. However, greater control over the tissue distribution of transgene expression is required to avoid potentially deleterious effects in non-target organs. In this regard, the liver is particularly at risk due to the high natural tropism of Ad for this organ, where dose limiting toxicity has been seen due to toxic transgene expression. We hypothesized that the cre/loxP system could be utilized to reduce unintended transgene expression at this site. This concept was tested using an Ad vector (AdLCLLL) carrying a reporter gene cassette in which the promoter and luciferase gene were flanked by LoxP sequences. Co-administration of this vector with a second vector carrying the cre recombinase gene in vitro and in vivo resulted in specific down-regulation of transgene expression. This novel approach thus has the potential to improve the safety of gene therapy strategies that rely upon the delivery of genes which may be hepatotoxic.

Original languageEnglish
Pages (from-to)1271-1275
Number of pages5
JournalGene therapy
Volume8
Issue number16
DOIs
StatePublished - Sep 4 2001
Externally publishedYes

Keywords

  • Adenovirus
  • Cre recombinase
  • Gene therapy
  • Liver

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