TY - JOUR
T1 - A novel murine segmental femoral graft model
AU - Tiyapatanaputi, Prarop
AU - Rubery, Paul T.
AU - Carmouche, Jonathan
AU - Schwarz, Edward M.
AU - O'Keefe, Regis J.
AU - Zhang, Xinping
N1 - Funding Information:
P.T. is supported by an unrestricted research fellowship from DePuy Inc./a Johnson and Johnson company. E.S. is supported by grants from The Musculoskeletal Transplant Foundation and The National Institute of Health (AR45971). R.J. is supported by The National Institute of Health (AR46545). P.R. is supported by Musculoskeletal Transplantation Foundation and X.Z. is supported by The Orthopedic Research Education Foundation and Musculoskeletal Transplant Foundation. The Authors thank Drs. D. Brooks and J. Gorczyca for assistance in the establishing the mouse model. We also thank J. Harvey for her assistance with histological work.
PY - 2004/11
Y1 - 2004/11
N2 - To further understand the cellular and molecular mechanisms underlying cortical bone graft healing, we have developed a novel mouse femur model that permits quantitative and molecular analysis of structural bone graft healing. A 4 mm mid-diaphyseal femoral segment was removed and replaced by either immediate implantation of a fresh autograft, a frozen, genetically identical isograft or a frozen allograft from a different strain of mouse, which was secured with a 22-gauge metal intramedullary pin. Healing was evaluated by radiology, histomorphometry, and in situ hybridization. Autograft repair occurred by endochondral bone formation at the host-graft junction and by intramembranous bone formation along the length of the graft bed at 2 weeks, with maturation and remodeling apparent by 4 weeks. Bone repair in allografts and isografts completely relied on endochondral bone formation at the host-graft cortical junction, with absence of periosteal bone formation along the length of the graft, suggesting that live periosteal cells from the donor tissue are necessary for this response. This small animal model of structural bone grafting can be used to evaluate tissue-engineered allografts and novel bone graft substitutes using quantitative and molecularly defined outcome measures.
AB - To further understand the cellular and molecular mechanisms underlying cortical bone graft healing, we have developed a novel mouse femur model that permits quantitative and molecular analysis of structural bone graft healing. A 4 mm mid-diaphyseal femoral segment was removed and replaced by either immediate implantation of a fresh autograft, a frozen, genetically identical isograft or a frozen allograft from a different strain of mouse, which was secured with a 22-gauge metal intramedullary pin. Healing was evaluated by radiology, histomorphometry, and in situ hybridization. Autograft repair occurred by endochondral bone formation at the host-graft junction and by intramembranous bone formation along the length of the graft bed at 2 weeks, with maturation and remodeling apparent by 4 weeks. Bone repair in allografts and isografts completely relied on endochondral bone formation at the host-graft cortical junction, with absence of periosteal bone formation along the length of the graft, suggesting that live periosteal cells from the donor tissue are necessary for this response. This small animal model of structural bone grafting can be used to evaluate tissue-engineered allografts and novel bone graft substitutes using quantitative and molecularly defined outcome measures.
KW - Allografts
KW - Autografts
KW - Isografts
UR - http://www.scopus.com/inward/record.url?scp=4844220921&partnerID=8YFLogxK
U2 - 10.1016/j.orthres.2004.03.017
DO - 10.1016/j.orthres.2004.03.017
M3 - Article
C2 - 15475206
AN - SCOPUS:4844220921
VL - 22
SP - 1254
EP - 1260
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
SN - 0736-0266
IS - 6
ER -