A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

Diane J. Aum, Ananth K. Vellimana, Itender Singh, Eric Milner, James W. Nelson, Byung Hee Han, Gregory J. Zipfel

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye. We found that ROX SE perfusion achieves excellent delineation of the cerebral vasculature, was qualitatively and quantitatively superior to India ink-gelatin casting for the assessment of cerebral vasospasm, permits outstanding immunohistochemical examination of non-vasospasm components of secondary brain injury, and is a more efficient and cost-effective experimental technique. ROX SE perfusion is therefore a novel and highly useful technique for studying cerebrovascular pathology following experimental SAH.

Original languageEnglish
Article number9126
JournalScientific reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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