A novel duplex ELISA method for quantitation of plasma proteins in areas of brain edema

Matthew C. Loftspring, Joseph F. Clark, Kenneth R. Wagner

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have developed a novel type of duplex enzyme-linked immunosorbent assay (ELISA) for the quantitation of the major plasma proteins, IgG and albumin, in edematous brain tissue. We test this duplex ELISA on our porcine intracerebral hemorrhage (ICH) model and show that it is as accurate and sensitive as independent single ELISAs. This method is useful as a marker of edema in brain tissue and the same design can be applied to other proteins and sample types.

Original languageEnglish
Pages (from-to)130-132
Number of pages3
JournalBrain Research
Volume1162
Issue number1
DOIs
StatePublished - Aug 8 2007

Keywords

  • ELISA
  • Edema
  • Intracerebral hemorrhage
  • Stroke

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