TY - JOUR
T1 - A novel approach to a bifunctional photosensitizer for tumor imaging and phototherapy
AU - Chen, Yihui
AU - Gryshuk, Amy
AU - Achilefu, Samuel
AU - Ohulchansky, Tymish
AU - Potter, William
AU - Zhong, Tuoxiu
AU - Morgan, Janet
AU - Chance, Britton
AU - Prasad, Paras N.
AU - Henderson, Barbara W.
AU - Oseroff, Allan
AU - Pandey, Ravindra K.
PY - 2005
Y1 - 2005
N2 - Optical imaging has attracted a great attention for studying molecular recognitions because minute fluorescent tracers can be detected in homogeneous and heterogeneous media with existing laboratory instruments. In our preliminary study, a clinically relevant photosensitizer (HPPH, a chlorophyll-a analog) was linked with a cyanine dye (with required photophysical characteristics but limited tumor selectivity), and the resulting conjugate was found to be an efficient tumor imaging (fluorescence imaging) and photosensitizing agent. Compared to HPPH, the presence of the cyanine dye moiety in the conjugate produced a significantly higher uptake in tumor than skin. At a therapeutic/imaging dose, the conjugate did not show any significant skin phototoxicity, a major drawback associated with most of the porphyrin-based photosensitizers. These results suggest that tumor-avid porphyrin-based compounds can be used as "vehicles" to deliver the desired fluorescent agent(s) to tumor. The development of tumor imaging or improved photodynamic therapy agent(s) by itself represents an important step, but a dual function agent (fluorescence imaging and photodynamic therapy) provides the potential for tumor detection and targeted photodynamic therapy, combining two modalities into a single cost-effective "see and treat" approach.
AB - Optical imaging has attracted a great attention for studying molecular recognitions because minute fluorescent tracers can be detected in homogeneous and heterogeneous media with existing laboratory instruments. In our preliminary study, a clinically relevant photosensitizer (HPPH, a chlorophyll-a analog) was linked with a cyanine dye (with required photophysical characteristics but limited tumor selectivity), and the resulting conjugate was found to be an efficient tumor imaging (fluorescence imaging) and photosensitizing agent. Compared to HPPH, the presence of the cyanine dye moiety in the conjugate produced a significantly higher uptake in tumor than skin. At a therapeutic/imaging dose, the conjugate did not show any significant skin phototoxicity, a major drawback associated with most of the porphyrin-based photosensitizers. These results suggest that tumor-avid porphyrin-based compounds can be used as "vehicles" to deliver the desired fluorescent agent(s) to tumor. The development of tumor imaging or improved photodynamic therapy agent(s) by itself represents an important step, but a dual function agent (fluorescence imaging and photodynamic therapy) provides the potential for tumor detection and targeted photodynamic therapy, combining two modalities into a single cost-effective "see and treat" approach.
UR - http://www.scopus.com/inward/record.url?scp=25444511317&partnerID=8YFLogxK
U2 - 10.1021/bc050177o
DO - 10.1021/bc050177o
M3 - Article
C2 - 16173807
AN - SCOPUS:25444511317
SN - 1043-1802
VL - 16
SP - 1264
EP - 1274
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
IS - 5
ER -