A novel actin-related protein is associated with daughter cell formation in Toxoplasma gondii

Jennifer L. Gordon, Wandy L. Beatty, L. David Sibley

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Cell division in Toxoplasma gondii occurs by an unusual budding mechanism termed endodyogeny, during which twin daughters are formed within the body of the mother cell. Cytokinesis begins with the coordinated assembly of the inner membrane complex (IMC), which surrounds the growing daughter cells. The IMC is compiled of both flattened membrane cisternae and subpellicular filaments composed of articulin-like proteins attached to underlying singlet microtubules. While proteins that comprise the elongating IMC have been described, little is known about its initial formation. Using Toxoplasma as a model system, we demonstrate that actin-like protein 1 (ALP1) is partially redistributed to the IMC at early stages in its formation. Immunoelectron microscopy localized ALP1 to a discrete region of the nuclear envelope, on transport vesicles, and on the nascent IMC of the daughter cells prior to the arrival of proteins such as IMC-1. The overexpression of ALP1 under the control of a strong constitutive promoter disrupted the formation of the daughter cell IMC, leading to delayed growth and defects in nuclear and apicoplast segregation. Collectively, these data suggest that ALP1 participates in the formation of daughter cell membranes during cell division in apicomplexan parasites.

Original languageEnglish
Pages (from-to)1500-1512
Number of pages13
JournalEukaryotic Cell
Volume7
Issue number9
DOIs
StatePublished - Sep 2008

Fingerprint

Dive into the research topics of 'A novel actin-related protein is associated with daughter cell formation in Toxoplasma gondii'. Together they form a unique fingerprint.

Cite this