A noninvasive reporter system to image adenoviral-mediated gene transfer to ovarian cancer xenografts

Tandra R. Chaudhuri, Buck E. Rogers, Donald J. Buchsbaum, James M. Mountz, Kurt R. Zinn

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Objective. Gene therapy trials for ovarian cancer would benefit from a noninvasive imaging modality to detect the location and extent of gene transfer. The human type 2 somatostatin receptor gene (hSSTr2) was evaluated as a reporter gene for imaging adenoviral (Ad) gene transfer to ovarian cancer. Methods. A replication-incompetent Ad vector encoding hSSTr2 (Ad-hSSTr2) was used to infect SKOV3.ip1 cells in vitro and tumors growing in nude mice. Gamma camera imaging detected uptake of 99m-Tc-P2045 (a somatostatin analogue) due to expressed hSSTr2. Results. Specific uptake of 99m-Tc-P2045 was imaged in Ad-hSSTr2-infected cells in vitro. Noninvasive in vivo imaging detected gene transfer to intraperitoneal tumors. Uptake of 99m-Tc-P2045 (percentage dose per gram of tumor) averaged 2.2 and 0.18 for Ad-hSSTr2-injected mice and controls, respectively. Conclusion. This study reports the first noninvasive imaging method for imaging gene transfer to ovarian cancer. A human gene therapy trial is planned.

Original languageEnglish
Pages (from-to)432-438
Number of pages7
JournalGynecologic oncology
Volume83
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

Keywords

  • 99m-Tc
  • Gene therapy
  • Imaging
  • Ovarian cancer

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